Detailed information of TA system TA188319

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4977864..4978466
• Replicon: chromosome
• Accession: NZ_CP068815
• Organism: Escherichia coli strain RIVM_C028620

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN49_RS23725
• Protein ID: WP_000897305.1
• Coordinates: 4978155..4978466 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN49_RS23720
• Protein ID: WP_000356397.1
• Coordinates: 4977864..4978154 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN49_RS23700	4974366..4975268	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN49_RS23705	4975265..4975900	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN49_RS23710	4975897..4976826	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN49_RS23715	4977041..4977259	-	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
JNN49_RS23720	4977864..4978154	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
JNN49_RS23725	4978155..4978466	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN49_RS23730	4978695..4979603	+	909	WP_001298596.1	alpha/beta hydrolase	-
JNN49_RS23735	4979667..4980608	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN49_RS23740	4980653..4981090	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JNN49_RS23745	4981087..4981959	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JNN49_RS23750	4981953..4982552	-	600	WP_001298594.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T188319 WP_000897305.1 NZ_CP068815:c4978466-4978155 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T188319 NZ_CP090400:838093-838371 [Vibrio cholerae]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT188319 WP_000356397.1 NZ_CP068815:c4978154-4977864 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT188319 NZ_CP090400:837812-838096 [Vibrio cholerae]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7