Detailed information of TA system TA188309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3953536..3954230
• Replicon: chromosome
• Accession: NZ_CP068815
• Organism: Escherichia coli strain RIVM_C028620

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: JNN49_RS18855
• Protein ID: WP_001263500.1
• Coordinates: 3953536..3953934 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: JNN49_RS18860
• Protein ID: WP_000554758.1
• Coordinates: 3953937..3954230 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN49_RS18830	3948901..3949359	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
JNN49_RS18835	3949620..3951077	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
JNN49_RS18840	3951134..3951748	-	615	WP_000602124.1	peptide chain release factor H	-
JNN49_RS18845	3951745..3952884	-	1140	WP_000521561.1	RNA ligase RtcB family protein	-
JNN49_RS18850	3953074..3953526	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
JNN49_RS18855	3953536..3953934	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN49_RS18860	3953937..3954230	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN49_RS18865	3954282..3955337	-	1056	WP_001226168.1	DNA polymerase IV	-
JNN49_RS18870	3955408..3956193	-	786	WP_000207578.1	putative lateral flagellar export/assembly protein LafU	-
JNN49_RS18875	3956165..3957877	+	1713	Protein_3701	flagellar biosynthesis protein FlhA	-
JNN49_RS18880	3958197..3958694	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	blaOXA-48	gmhA/lpcA	3953536..3995762	42226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T188309 WP_001263500.1 NZ_CP068815:c3953934-3953536 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T188309 NZ_CP090398:746162-746647 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT188309 WP_000554758.1 NZ_CP068815:c3954230-3953937 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT188309 NZ_CP090398:745872-746165 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3