Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4215800..4216494 Replicon chromosome
Accession NZ_CP068814
Organism Escherichia coli strain RIVM_C028613

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag JNN56_RS20350 Protein ID WP_001263491.1
Coordinates 4215800..4216198 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag JNN56_RS20355 Protein ID WP_000554755.1
Coordinates 4216201..4216494 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN56_RS20320 4210969..4212213 - 1245 WP_000189541.1 esterase FrsA -
- 4211629..4211709 - 81 NuclAT_11 - -
- 4211629..4211709 - 81 NuclAT_11 - -
- 4211629..4211709 - 81 NuclAT_11 - -
- 4211629..4211709 - 81 NuclAT_11 - -
JNN56_RS20325 4212305..4212763 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
JNN56_RS20330 4213024..4214481 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
JNN56_RS20335 4214538..4214890 - 353 Protein_3983 peptide chain release factor H -
JNN56_RS20340 4214886..4215092 - 207 Protein_3984 RtcB family protein -
JNN56_RS20345 4215338..4215790 - 453 WP_019842500.1 GNAT family N-acetyltransferase -
JNN56_RS20350 4215800..4216198 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JNN56_RS20355 4216201..4216494 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JNN56_RS20360 4216546..4217601 - 1056 WP_001550655.1 DNA polymerase IV -
JNN56_RS20365 4217672..4218457 - 786 WP_019842510.1 putative lateral flagellar export/assembly protein LafU -
JNN56_RS20370 4218429..4220141 + 1713 Protein_3990 flagellar biosynthesis protein FlhA -
JNN56_RS20375 4220246..4220524 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JNN56_RS20380 4220517..4220873 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4205736..4216494 10758


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T188278 WP_001263491.1 NZ_CP068814:c4216198-4215800 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T188278 NZ_CP090390:53925-54407 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT188278 WP_000554755.1 NZ_CP068814:c4216494-4216201 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT188278 NZ_CP090390:53671-53937 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References