Detailed information of TA system TA188260

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1298877..1299604
• Replicon: chromosome
• Accession: NZ_CP068814
• Organism: Escherichia coli strain RIVM_C028613

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: JNN56_RS06220
• Protein ID: WP_000547555.1
• Coordinates: 1298877..1299188 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN56_RS06225
• Protein ID: WP_000126294.1
• Coordinates: 1299185..1299604 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN56_RS06195	1294812..1296521	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
JNN56_RS06200	1296531..1297073	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
JNN56_RS06205	1297073..1297840	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
JNN56_RS06210	1297837..1298247	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
JNN56_RS06215	1298240..1298710	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
JNN56_RS06220	1298877..1299188	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
JNN56_RS06225	1299185..1299604	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
JNN56_RS06230	1299683..1301107	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
JNN56_RS06235	1301116..1302573	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
JNN56_RS06240	1302833..1303843	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
JNN56_RS06245	1303992..1304519	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T188260 WP_000547555.1 NZ_CP068814:1298877-1299188 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T188260 NZ_CP090386:c995456-995154 [Vibrio cholerae]
ATGGTTGAAATAATTTGGACGGAGCCGGCTCTATCCGACCTGAATGATATTGCTGAATATATCGCGCTTGAAAATGTCGT
AGCTGCTAAACAACTGGTGCAAACCGTTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAGAGTCTGGGCGCGTCCCTC
CAGAACTAGAACACCTCAATTATCGTGAAGTCGTTGTAAATCCGTGTCGTGTTTTCTACAAGTATGATGATGCAAAGGTT
CGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT188260 WP_000126294.1 NZ_CP068814:1299185-1299604 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT188260 NZ_CP090386:c995701-995444 [Vibrio cholerae]
ATGAAAGTAGAACTTGTGACGTCACTAAAACGTCAGGCCACTAAGATCCTTGCCGATCTTCATGATACGAAAGAGCCAGT
ATTGATAACTGAGCACGGAAAACCGTCGGCTTACCTTATTGATGTAGACGACTATGAATTTATGCAAAATCGTTTAGCGA
TCCTGGAAGGTATTGCTCGCGGAGAGCGAGCTTTAGCGGATGGCAAAGTGGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGTTGAAATAA

Structures

Toxin

• AlphaFold DB: V0YLE2