Detailed information of TA system TA188252

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5027557..5028159
• Replicon: chromosome
• Accession: NZ_CP068813
• Organism: Escherichia coli strain RIVM_C029033

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4Y9BEM6
• Locus tag: JNN62_RS24180
• Protein ID: WP_059332311.1
• Coordinates: 5027848..5028159 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN62_RS24175
• Protein ID: WP_000356395.1
• Coordinates: 5027557..5027847 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN62_RS24140	5023181..5024083	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN62_RS24145	5024080..5024715	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN62_RS24150	5024712..5025641	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN62_RS24155	5025823..5026065	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
JNN62_RS24160	5026284..5026502	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN62_RS24165	5026921..5027199	-	279	WP_001315112.1	hypothetical protein	-
JNN62_RS24170	5027251..5027472	-	222	WP_001550354.1	hypothetical protein	-
JNN62_RS24175	5027557..5027847	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
JNN62_RS24180	5027848..5028159	-	312	WP_059332311.1	hypothetical protein	Toxin
JNN62_RS24185	5028388..5029296	+	909	WP_001550353.1	alpha/beta hydrolase	-
JNN62_RS24190	5029464..5031278	-	1815	WP_001550352.1	hypothetical protein	-
JNN62_RS24195	5031694..5032635	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN62_RS24200	5032680..5033117	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12212.25 Da
• Isoelectric Point: 10.0241

>T188252 WP_059332311.1 NZ_CP068813:c5028159-5027848 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPHYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T188252 NZ_CP090384:c34577-34290 [Acinetobacter towneri]
ATGAAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCACTGAGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAA
AAGTAAAAGGCGTACTAATGCTGTTAGTAGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAGGTAACTAAAGCGGAGCGAGATAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT188252 WP_000356395.1 NZ_CP068813:c5027847-5027557 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT188252 NZ_CP090384:c34303-33989 [Acinetobacter towneri]
ATGAGCATGGTTAGATACTCACGCAAAGAACTGAATGAAAAATTTAGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGTAATGCTATTCGTC
ATGGGCAATTTTATCGTCCAGTGAAGCAACAGACTTCCATCCGTTTAGATGCTGATGTACTTGCATGGTTTAAAGCTCAA
GGTAAAGGCTATCAAACACGAATGAATGAAATTCTGCGTGAAGCAATGTTAGAAGATTTAAAAAATCATCGATAA

Structures

Toxin

• AlphaFold DB: A0A4Y9BEM6