Detailed information of TA system TA188248

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4142994..4143688
• Replicon: chromosome
• Accession: NZ_CP068813
• Organism: Escherichia coli strain RIVM_C029033

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: JNN62_RS20035
• Protein ID: WP_001263491.1
• Coordinates: 4142994..4143392 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: JNN62_RS20040
• Protein ID: WP_000554755.1
• Coordinates: 4143395..4143688 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN62_RS20005	4138163..4139407	-	1245	WP_000189541.1	esterase FrsA	-
-	4138823..4138903	-	81	NuclAT_10	-	-
-	4138823..4138903	-	81	NuclAT_10	-	-
-	4138823..4138903	-	81	NuclAT_10	-	-
-	4138823..4138903	-	81	NuclAT_10	-	-
JNN62_RS20010	4139499..4139957	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JNN62_RS20015	4140218..4141675	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JNN62_RS20020	4141732..4142084	-	353	Protein_3920	peptide chain release factor H	-
JNN62_RS20025	4142080..4142286	-	207	Protein_3921	RtcB family protein	-
JNN62_RS20030	4142532..4142984	-	453	WP_019842500.1	GNAT family N-acetyltransferase	-
JNN62_RS20035	4142994..4143392	-	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN62_RS20040	4143395..4143688	-	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN62_RS20045	4143740..4144795	-	1056	WP_001550655.1	DNA polymerase IV	-
JNN62_RS20050	4144866..4145651	-	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
JNN62_RS20055	4145623..4147335	+	1713	Protein_3927	flagellar biosynthesis protein FlhA	-
JNN62_RS20060	4147440..4147718	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
JNN62_RS20065	4147711..4148067	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4132930..4143688	10758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T188248 WP_001263491.1 NZ_CP068813:c4143392-4142994 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T188248 NZ_CP090382:c1689935-1689531 [Acinetobacter towneri]
ATGTTGAAATATATGCTGGACACCAACATTGTAATTTATACAATGAAGCAAAGACCGATTGAAGTAAAAGAAAAATTTAA
TGCGGTTTCAACTCAACTGTGTATCAGTAGCATTAGTGTGGCAGAATTAATTTTCGGTGCAGAAAATAGCCAAACGCCTG
AAAAAAACTTAAAGATCATCGAAAACTTTTTATCACGCTTACAAATTTTGGATTATGGTGTAGATGCTGCAATTCAATAC
GGTAATATCAAGGCACATTTAAAGAAAATAGGCACACCTATTGGTGAGAATGATTTACATATTGCTGCTCATGCTCGAAG
TAAAGGATTGATACTCGTTACGAATAATACAAAAGAGTTTGAGCGAGTGCCTGCTTTACAAATTGAAAATTGGGTCAATG
GATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT188248 WP_000554755.1 NZ_CP068813:c4143688-4143395 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT188248 NZ_CP090382:c1690163-1689936 [Acinetobacter towneri]
ATGCTGACTTCTGTTTTTATGAATAATCGTACCCAAGCCGTGCGTTTACCCAGTATTTACCGCTTTTCTGAATCCACTCA
AAAAGTCTTTGTCCGTATGGTGGGAGTGGAACGTGTGATTAGTCCCGTAGAACAAGTGTGGGATAGCTTTTTTGATGATC
CTGATCTACAACTTTCTGCTGATTTTGAACGTGGTGAGCAAATCTCAACAGAACGAGAAGCATTCTAA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5