Detailed information of TA system TA188220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4081615..4082309
• Replicon: chromosome
• Accession: NZ_CP068812
• Organism: Escherichia coli strain RIVM_C029020

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: JNN61_RS19420
• Protein ID: WP_001263491.1
• Coordinates: 4081911..4082309 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: JNN61_RS19415
• Protein ID: WP_000554755.1
• Coordinates: 4081615..4081908 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN61_RS19390	4077236..4077592	-	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-
JNN61_RS19395	4077585..4077863	-	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
JNN61_RS19400	4077968..4079680	-	1713	Protein_3784	flagellar biosynthesis protein FlhA	-
JNN61_RS19405	4079652..4080437	+	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
JNN61_RS19410	4080508..4081563	+	1056	WP_001550655.1	DNA polymerase IV	-
JNN61_RS19415	4081615..4081908	+	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN61_RS19420	4081911..4082309	+	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN61_RS19425	4082319..4082771	+	453	WP_019842500.1	GNAT family N-acetyltransferase	-
JNN61_RS19430	4083017..4083223	+	207	Protein_3790	RtcB family protein	-
JNN61_RS19435	4083219..4083571	+	353	Protein_3791	peptide chain release factor H	-
JNN61_RS19440	4083628..4085085	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JNN61_RS19445	4085346..4085804	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JNN61_RS19450	4085896..4087140	+	1245	WP_000189541.1	esterase FrsA	-
-	4086400..4086480	+	81	NuclAT_11	-	-
-	4086400..4086480	+	81	NuclAT_11	-	-
-	4086400..4086480	+	81	NuclAT_11	-	-
-	4086400..4086480	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T188220 WP_001263491.1 NZ_CP068812:4081911-4082309 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T188220 NZ_CP090366:c2802087-2801794 [Stutzerimonas kunmingensis]
GTGCCACGACTGATCGTCACTGAAGGCGCGGCGAAGGGCCTGGAGCGTTGCCGGCGATTTCTCTCCGATAAAGATCCGCA
GATCGCACGCCGTGCTGCACAGGCAATCGAGCGCCAATTCGCTCGATTGGAGGAAAGTCCGGAAGTAGGGCGTCCATTTC
CAGATCTGCCAGAGCTGCGTGAGCTCATCATCGAGTTTGGTGACTCAGGCTACGTCGCGCTGTACCGCTATGAGCGCGCA
GATGACACCGCCTATGTCTTGGCCTTTCGCCATCAAAAAGAAGCTGGCTACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT188220 WP_000554755.1 NZ_CP068812:4081615-4081908 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT188220 NZ_CP090366:c2802347-2802075 [Stutzerimonas kunmingensis]
ATGGCCACTTCCATCAAAATTGACGACGATCTGAAAGGTCGCATTCAGCATTTGGCTGGTTTGCGGCAGCGCTCATCTCA
CTGGATCATGCGTGAGGCGATCACCCAGTACGTCGAACGTGAGGAGGCCCGTGAGAGCTTCAAACAAGAAGCGCTGGCAT
CTTGGGCTGCATACCAGGAAACCGGCCAGCATTTGACCGGCCAGGAGGCTCGCACCTGGCTTGGTAGTTGGGGTACAGAT
GCTGAGGCAGAGTTACCCAAGTGCCACGACTGA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5