Detailed information of TA system TA188213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3099525..3100127
• Replicon: chromosome
• Accession: NZ_CP068812
• Organism: Escherichia coli strain RIVM_C029020

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN61_RS14735
• Protein ID: WP_000897305.1
• Coordinates: 3099525..3099836 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN61_RS14740
• Protein ID: WP_000356395.1
• Coordinates: 3099837..3100127 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN61_RS14715	3094567..3095004	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JNN61_RS14720	3095049..3095990	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN61_RS14725	3096406..3098220	+	1815	WP_001550352.1	hypothetical protein	-
JNN61_RS14730	3098388..3099296	-	909	WP_001550353.1	alpha/beta hydrolase	-
JNN61_RS14735	3099525..3099836	+	312	WP_000897305.1	hypothetical protein	Toxin
JNN61_RS14740	3099837..3100127	+	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
JNN61_RS14745	3100212..3100433	+	222	WP_001550354.1	hypothetical protein	-
JNN61_RS14750	3100485..3100763	+	279	WP_001315112.1	hypothetical protein	-
JNN61_RS14755	3101182..3101400	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN61_RS14760	3101619..3101861	+	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
JNN61_RS14765	3102043..3102972	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN61_RS14770	3102969..3103604	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN61_RS14775	3103601..3104503	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T188213 WP_000897305.1 NZ_CP068812:3099525-3099836 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T188213 NZ_CP090356:c4131773-4131420 [Pantoea ananatis]
GTGTGGCCGATTAAAACAACGGATACGTTTGACTGCTGGTTTACCTCGCTTAGCGATATCGACAGGGCTGACGTGCTTGC
AGCATTGTTGGTCCTGCGGGAGAAGGGGCCGGGTTTGTCCAGACCTTATGCCGATACGCTCAAAGGCTCCCGCTATAGCA
ACATGAAAGAACGACGTATCCAGAGCCGGGGCGATCCTTTACGGGCATTTTTTGCCTTTGACCCGGCCCGAACCGGCATT
GTGCTTTGTGCCGGAAATAAGGTCGGCAATGAAAAACGTTTTTACGACGCGATGCTCCCGGTTGCTGACCGGGAATTCAC
GAACTGGCTTAACGCATTAAAAAATAAGGAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT188213 WP_000356395.1 NZ_CP068812:3099837-3100127 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT188213 NZ_CP090356:c4131415-4131113 [Pantoea ananatis]
ATGGGCAGAACGCTGGAACAGCTTATTGCGGATGAAAAAAATGACGTCGTGGATGAAGCTCAGGCGATGGCGACGGACAT
CCTGCTCAATATTCACCTTGCCGAACTGCGTGAGAAAGTACAAAAAACGCAGGTAGAAATGGCGCAGGCGCTGGGCATCA
AACAACCTACCGTTGCAGGAATGGAAAAACCGGGGCGCGACCTGAAGCTGTCCACGCTCAAACGCTATGTTGAAGCGACC
GGTGGCAAGCTTCGCCTTGATGTTGAACTGCCGGATGGTTCTCACTACGCTTTTGTGCTTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7