Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2166544..2167343 Replicon chromosome
Accession NZ_CP068812
Organism Escherichia coli strain RIVM_C029020

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag JNN61_RS10250 Protein ID WP_000347270.1
Coordinates 2166879..2167343 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag JNN61_RS10245 Protein ID WP_001551693.1
Coordinates 2166544..2166879 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN61_RS10230 2162329..2163099 - 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -
JNN61_RS10235 2163115..2164449 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
JNN61_RS10240 2164824..2166395 + 1572 WP_033878411.1 galactarate dehydratase -
JNN61_RS10245 2166544..2166879 + 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JNN61_RS10250 2166879..2167343 + 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JNN61_RS10255 2167398..2168207 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JNN61_RS10260 2168456..2169736 + 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
JNN61_RS10265 2169759..2170232 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JNN61_RS10270 2170243..2171022 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JNN61_RS10275 2171012..2171890 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JNN61_RS10280 2171908..2172342 + 435 WP_000948837.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2157782..2167343 9561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T188210 WP_000347270.1 NZ_CP068812:2166879-2167343 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

>T188210 NZ_CP090356:c3835008-3834911 [Pantoea ananatis]
GCAAGGCGAAAGCCTCTATTAATGCCAACTTTTAGCGCACGGCTCCTTGAGAGCCATTTCCCTGGACCGAATACAGGAAT
CGTGTTCGGTCTTTTTTT

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT188210 WP_001551693.1 NZ_CP068812:2166544-2166879 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT188210 NZ_CP090356:3834911-3835008 [Pantoea ananatis]
AAAAAAAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTCAAGGAGCCGTGCGCTAAAAGTTGGCATTAA
TAGAGGCTTTCGCCTTGC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References