Detailed information of TA system TA188206

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1624022..1624749
• Replicon: chromosome
• Accession: NZ_CP068812
• Organism: Escherichia coli strain RIVM_C029020

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: JNN61_RS07690
• Protein ID: WP_000547555.1
• Coordinates: 1624438..1624749 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN61_RS07685
• Protein ID: WP_000126294.1
• Coordinates: 1624022..1624441 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN61_RS07665	1619107..1619634	-	528	WP_001078777.1	electron transport protein HydN	-
JNN61_RS07670	1619783..1620793	-	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
JNN61_RS07675	1621053..1622510	+	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
JNN61_RS07680	1622519..1623943	+	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
JNN61_RS07685	1624022..1624441	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
JNN61_RS07690	1624438..1624749	-	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
JNN61_RS07695	1624916..1625386	-	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
JNN61_RS07700	1625379..1625789	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
JNN61_RS07705	1625786..1626553	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
JNN61_RS07710	1626553..1627095	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
JNN61_RS07715	1627105..1628814	-	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T188206 WP_000547555.1 NZ_CP068812:c1624749-1624438 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T188206 NZ_CP090355:c4246907-4246623 [Pseudomonas kribbensis]
ATGCAGATTAAGTGGCGACCCAAGGCTCGGGTCGAACTCTCGAAAATACTGAAGTACATTGGCGAGCGAAATTTTGTAGC
GGCGACAGCACTTCACAAGTCAGTCGTAAAAACTACATCTGCGCTTCCTTGGCATCCACACCTGTATCGCAAAGGTCGCT
CTCCCGGCACTCGGGAGATTGTGGTAAGCCCGAACTATCTCGTCGTTTATCAAGTGGCGGACCGAATTGAGATTGTGAGC
ATCTTGCATGCGCGTCAGGAATACCCTCGACGCGACCCCAGTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT188206 WP_000126294.1 NZ_CP068812:c1624441-1624022 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT188206 NZ_CP090355:c4247103-4246897 [Pseudomonas kribbensis]
ATGATCGACCCGTCTTTGATCGACCCACTGTGCCCGGTTGTTTCGGATCTTGCTCCTGAGGAACAGGCTGCGAGTTATGA
CCGTTGGTTTCGGGCCGAAGTACAGGCCTCTCTCGATGATCCGCGTCCGAGTATTCCCCATGAGCAAGTGATGGCGGAAA
TAAACGCCTTGATGGAATCAATGCGCAAAAGCGCTGATGCAGATTAA

Structures

Toxin

• AlphaFold DB: V0YLE2