Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 907755..908257 Replicon chromosome
Accession NZ_CP068812
Organism Escherichia coli strain RIVM_C029020

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JNN61_RS04450 Protein ID WP_000767829.1
Coordinates 907755..908009 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JNN61_RS04455 Protein ID WP_001259255.1
Coordinates 908006..908257 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN61_RS04420 902770..902997 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
JNN61_RS04425 903011..904069 - 1059 WP_023910197.1 transport protein YeeE -
JNN61_RS04430 904248..905606 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JNN61_RS04435 905596..905658 - 63 WP_010723108.1 membrane protein YoeI -
JNN61_RS04440 905873..906802 - 930 WP_000803351.1 LysR family transcriptional regulator -
JNN61_RS04445 906848..907672 - 825 WP_202849972.1 SDR family oxidoreductase -
JNN61_RS04450 907755..908009 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JNN61_RS04455 908006..908257 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JNN61_RS04460 908540..908590 + 51 WP_001364200.1 his operon leader peptide -
JNN61_RS04465 908736..909635 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
JNN61_RS04470 909641..910945 + 1305 WP_001559366.1 histidinol dehydrogenase -
JNN61_RS04475 910942..912012 + 1071 WP_000108970.1 histidinol-phosphate transaminase -
JNN61_RS04480 912012..913079 + 1068 WP_001563933.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T188205 WP_000767829.1 NZ_CP068812:c908009-907755 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T188205 NZ_CP090355:c2294378-2294064 [Pseudomonas kribbensis]
ATGATTTTTATTGAGACTCGGATCTTTACCCGTCGAGTAAAAGAGCTGCTGGACGATGACACTTACGCCGCTTTTCAGAA
GCAACTGGTTGTAAGTCCTTCGATTGGCGATGTAATTGAAGGCACAGGCGGGATCCGAAAAACCCGAATCGCAGCGAAAG
GACACGGCAAGCGAGGCGGAGCACGGGTGATCTACTACCACTTCGTTTCGGCGTCACAAATCGGGTTATTGATGATCTAC
CCTAAAAATGAACAACACGATCTGTCCAGCGATGAGCGTAAAGCACTCAAGGTCTTGATCGAAAAATGGAGATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT188205 WP_001259255.1 NZ_CP068812:c908257-908006 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT188205 NZ_CP090355:c2294061-2293774 [Pseudomonas kribbensis]
ATGAGCAAATTTTTTGATGAGCTGATGGAAAGTGTGCAAGAGATGGACGAGATTGTACGAGGCCAGCGCGCACCTTCTCG
CGAATTCCACGTTGATGCTTTGCAGGTCAAAGAGATTCGTAAAGCTACCGGTTTGACCCAGGCCAAATTTGCGGCACTGA
TCGACGTCCAGCTCGGCACTCTACGAAATTGGGAACAGGGTCGTCGCGAGCCTACCGGGCCGGCAAAAGCTTTGCTCAAG
GCCATTCATAACGACCCGGTTCATGTTTTGAATGCATTGGCCCATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References