Detailed information of TA system TA188190

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 121686..122057
• Replicon: chromosome
• Accession: NZ_CP068812
• Organism: Escherichia coli strain RIVM_C029020

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: JNN61_RS00600
• Protein ID: WP_001317028.1
• Coordinates: 121863..122057 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	121686..121864 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN61_RS00575	117641..119014	+	1374	WP_001551034.1	ATP-dependent RNA helicase DbpA	-
JNN61_RS00580	119143..120078	-	936	WP_000662472.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
JNN61_RS00585	120130..121365	-	1236	WP_001551035.1	site-specific integrase	-
JNN61_RS00590	121367..121582	-	216	WP_000079604.1	excisionase XisR	-
JNN61_RS00595	121682..121870	-	189	WP_001551036.1	DUF1187 family protein	-
-	121686..121864	+	179	NuclAT_0	-	Antitoxin
-	121686..121864	+	179	NuclAT_0	-	Antitoxin
-	121686..121864	+	179	NuclAT_0	-	Antitoxin
-	121686..121864	+	179	NuclAT_0	-	Antitoxin
JNN61_RS00600	121863..122057	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
JNN61_RS00605	122114..122923	-	810	WP_001551037.1	recombination protein RecT	-
JNN61_RS00610	122916..125567	-	2652	WP_001551038.1	exodeoxyribonuclease VIII	-
JNN61_RS00615	125669..125944	-	276	WP_001314664.1	hypothetical protein	-
JNN61_RS00620	126019..126189	-	171	WP_001551041.1	phage protein	-
JNN61_RS00625	126189..126410	-	222	WP_000560221.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	120130..167544	47414

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T188190 WP_001317028.1 NZ_CP068812:c122057-121863 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T188190 NZ_CP090345:c111529-111380 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT188190 NZ_CP068812:121686-121864 [Escherichia coli]
GAGGACTGAAGTCTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGGCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin