Detailed information of TA system TA188189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5007147..5007749
• Replicon: chromosome
• Accession: NZ_CP068811
• Organism: Escherichia coli strain RIVM_C028568

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN42_RS24090
• Protein ID: WP_000897305.1
• Coordinates: 5007438..5007749 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN42_RS24085
• Protein ID: WP_000356395.1
• Coordinates: 5007147..5007437 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN42_RS24050	5002771..5003673	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN42_RS24055	5003670..5004305	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN42_RS24060	5004302..5005231	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN42_RS24065	5005413..5005655	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
JNN42_RS24070	5005874..5006092	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN42_RS24075	5006511..5006789	-	279	WP_001315112.1	hypothetical protein	-
JNN42_RS24080	5006841..5007062	-	222	WP_001550354.1	hypothetical protein	-
JNN42_RS24085	5007147..5007437	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
JNN42_RS24090	5007438..5007749	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN42_RS24095	5007978..5008886	+	909	WP_001550353.1	alpha/beta hydrolase	-
JNN42_RS24100	5009054..5010868	-	1815	WP_001550352.1	hypothetical protein	-
JNN42_RS24105	5011284..5012225	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN42_RS24110	5012270..5012707	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T188189 WP_000897305.1 NZ_CP068811:c5007749-5007438 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T188189 NZ_CP090344:c108522-108106 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT188189 WP_000356395.1 NZ_CP068811:c5007437-5007147 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT188189 NZ_CP090344:c108749-108519 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7