Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1952578..1953080 Replicon chromosome
Accession NZ_CP068811
Organism Escherichia coli strain RIVM_C028568

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JNN42_RS09205 Protein ID WP_000767829.1
Coordinates 1952826..1953080 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JNN42_RS09200 Protein ID WP_001259255.1
Coordinates 1952578..1952829 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN42_RS09175 1947756..1948823 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
JNN42_RS09180 1948823..1949893 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
JNN42_RS09185 1949890..1951194 - 1305 WP_039267630.1 histidinol dehydrogenase -
JNN42_RS09190 1951200..1952099 - 900 WP_000131789.1 ATP phosphoribosyltransferase -
JNN42_RS09195 1952245..1952295 - 51 WP_071593552.1 his operon leader peptide -
JNN42_RS09200 1952578..1952829 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JNN42_RS09205 1952826..1953080 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JNN42_RS09210 1953163..1953987 + 825 WP_000754737.1 SDR family oxidoreductase -
JNN42_RS09215 1954033..1954962 + 930 WP_000803351.1 LysR family transcriptional regulator -
JNN42_RS09220 1955177..1955239 + 63 WP_010723108.1 membrane protein YoeI -
JNN42_RS09225 1955229..1956587 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JNN42_RS09230 1956766..1957824 + 1059 WP_023568741.1 transport protein YeeE -
JNN42_RS09235 1957838..1958065 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T188166 WP_000767829.1 NZ_CP068811:1952826-1953080 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T188166 NZ_CP090316:c1951235-1950933 [Xylella fastidiosa subsp. morus]
ATGGAGAAAGGCACACCCCACTACAAGCTACCAGTCGTCAAAGCCTTGGTGAAAGCTGGCAAGGTCAGGGCTACAGCGAC
CGCCTACCAAGGAGCACGTGAGCTTGGCATCAAAGATTTAACTGGAATGTGCGCCGTTGTCTTGGCGCTCACGTCTGCCG
ACTTCTACAAGAGTATGACGACCTACACGGATCACACATTATTCCAGGATGTGTACCACGCCAAGACGGCAAGCGGTGAT
GAGGTGTACCTGAAGTTAACTGTCATTGATGACGTGTTGATTGTTTCCTTCAAGGAGTTATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT188166 WP_001259255.1 NZ_CP068811:1952578-1952829 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT188166 NZ_CP090316:c1950930-1950529 [Xylella fastidiosa subsp. morus]
ATGAGATGCCCATGCTGCGGCGCGGCAGAACTGATCCACGACACGCGCGACATGCTTTACACCTACAAGAGCGAAACTAC
CAGTATCCCAACGGTGACCGGCGACTTCTGCCCGGCTTGTGGCGAAGTCGTCTTGGACCGTGAACATGGTGATCGTTACA
GCGAATTGGTCGGCCTGTTCCAACGTCAGGTGAATTCGGCCTACGTCGATCCTGGTTACATTACCAAGGTACGCCGGAAG
CTTGATCTCGACCAGCGGCAAGCTGCCGAACTGTTCGGTGGTGGTGTTAATGCTTTCTCACGTTACGAGAATGGTAAAAC
CAAGCCGCCGCTGTCTTTGGTTAAGTTATTCAAGCTTCTGGATCGCCACCCCGATTTGCTCAACGAAGTGAAGAGTTCCT
AA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References