Detailed information of TA system TA188166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1952578..1953080
• Replicon: chromosome
• Accession: NZ_CP068811
• Organism: Escherichia coli strain RIVM_C028568

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: JNN42_RS09205
• Protein ID: WP_000767829.1
• Coordinates: 1952826..1953080 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: JNN42_RS09200
• Protein ID: WP_001259255.1
• Coordinates: 1952578..1952829 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN42_RS09175	1947756..1948823	-	1068	WP_000080093.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
JNN42_RS09180	1948823..1949893	-	1071	WP_000108943.1	histidinol-phosphate transaminase	-
JNN42_RS09185	1949890..1951194	-	1305	WP_039267630.1	histidinol dehydrogenase	-
JNN42_RS09190	1951200..1952099	-	900	WP_000131789.1	ATP phosphoribosyltransferase	-
JNN42_RS09195	1952245..1952295	-	51	WP_071593552.1	his operon leader peptide	-
JNN42_RS09200	1952578..1952829	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
JNN42_RS09205	1952826..1953080	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
JNN42_RS09210	1953163..1953987	+	825	WP_000754737.1	SDR family oxidoreductase	-
JNN42_RS09215	1954033..1954962	+	930	WP_000803351.1	LysR family transcriptional regulator	-
JNN42_RS09220	1955177..1955239	+	63	WP_010723108.1	membrane protein YoeI	-
JNN42_RS09225	1955229..1956587	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
JNN42_RS09230	1956766..1957824	+	1059	WP_023568741.1	transport protein YeeE	-
JNN42_RS09235	1957838..1958065	+	228	WP_000985273.1	sulfurtransferase TusA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T188166 WP_000767829.1 NZ_CP068811:1952826-1953080 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T188166 NZ_CP090316:c1951235-1950933 [Xylella fastidiosa subsp. morus]
ATGGAGAAAGGCACACCCCACTACAAGCTACCAGTCGTCAAAGCCTTGGTGAAAGCTGGCAAGGTCAGGGCTACAGCGAC
CGCCTACCAAGGAGCACGTGAGCTTGGCATCAAAGATTTAACTGGAATGTGCGCCGTTGTCTTGGCGCTCACGTCTGCCG
ACTTCTACAAGAGTATGACGACCTACACGGATCACACATTATTCCAGGATGTGTACCACGCCAAGACGGCAAGCGGTGAT
GAGGTGTACCTGAAGTTAACTGTCATTGATGACGTGTTGATTGTTTCCTTCAAGGAGTTATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT188166 WP_001259255.1 NZ_CP068811:1952578-1952829 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT188166 NZ_CP090316:c1950930-1950529 [Xylella fastidiosa subsp. morus]
ATGAGATGCCCATGCTGCGGCGCGGCAGAACTGATCCACGACACGCGCGACATGCTTTACACCTACAAGAGCGAAACTAC
CAGTATCCCAACGGTGACCGGCGACTTCTGCCCGGCTTGTGGCGAAGTCGTCTTGGACCGTGAACATGGTGATCGTTACA
GCGAATTGGTCGGCCTGTTCCAACGTCAGGTGAATTCGGCCTACGTCGATCCTGGTTACATTACCAAGGTACGCCGGAAG
CTTGATCTCGACCAGCGGCAAGCTGCCGAACTGTTCGGTGGTGGTGTTAATGCTTTCTCACGTTACGAGAATGGTAAAAC
CAAGCCGCCGCTGTCTTTGGTTAAGTTATTCAAGCTTCTGGATCGCCACCCCGATTTGCTCAACGAAGTGAAGAGTTCCT
AA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0