Detailed information of TA system TA188158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5076313..5076915
• Replicon: chromosome
• Accession: NZ_CP068810
• Organism: Escherichia coli strain RIVM_C028536

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN60_RS24475
• Protein ID: WP_000897305.1
• Coordinates: 5076604..5076915 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN60_RS24470
• Protein ID: WP_000356395.1
• Coordinates: 5076313..5076603 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN60_RS24435	5071937..5072839	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN60_RS24440	5072836..5073471	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN60_RS24445	5073468..5074397	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN60_RS24450	5074579..5074821	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
JNN60_RS24455	5075040..5075258	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN60_RS24460	5075677..5075955	-	279	WP_001315112.1	hypothetical protein	-
JNN60_RS24465	5076007..5076228	-	222	WP_001550354.1	hypothetical protein	-
JNN60_RS24470	5076313..5076603	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
JNN60_RS24475	5076604..5076915	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN60_RS24480	5077144..5078052	+	909	WP_001550353.1	alpha/beta hydrolase	-
JNN60_RS24485	5078220..5080034	-	1815	WP_001550352.1	hypothetical protein	-
JNN60_RS24490	5080450..5081391	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN60_RS24495	5081436..5081873	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T188158 WP_000897305.1 NZ_CP068810:c5076915-5076604 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T188158 NZ_CP090316:503969-504472 [Xylella fastidiosa subsp. morus]
ATGAAATACGCAGGGGATCGTGGTGATCCTTACCTGGACAGCGAAACAGGTGTTCTTCGTAATCTCCTTGGAATCAGGGA
TCAGGGGTGGCTCGATAAAATAGAGTCCACCCTTTCTTTTTTGCGAACCAGCGAATTGCGTGAGCGACCCGTAAAAGGCA
AATTTGATCTAGCGCATTTACAGGAAATCCATAATCGTCTTTTCCAGGATGTGTACGACTGGGCGGGTCAAATCCGTCAA
GTTGAAATCTCGAAAGGCAACACGATGTTTGCCCAGCAGATTGCGATTCAGAGTGCGGCGCAGCAGATCTTTGGGCAGCT
TGCTAAAGAGCGATTTTTGTGTGGCCTTGATGCTGAAGAATTCAGCAAACGGGCAGGTGATTATCTTGGTGAAATTAATG
TACTTCACCCATTCCGCGAGGGAAATGGCAGGACGCAGCGGGAATTCATCGCGCAATTGGCTCAGCGTGCAGGTTACCGG
ATCGACTGGGGCGTGGTCAGCTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT188158 WP_000356395.1 NZ_CP068810:c5076603-5076313 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT188158 NZ_CP090316:503784-503972 [Xylella fastidiosa subsp. morus]
ATGCTGACAGATCATGAAAAAGCTGAACGCCGTGCTGTGGTGCAAAGCGCACTCGCAAGCCAACGCATTGAGGGGTTAGA
GCCGGATGCGCAAGCCGTTGTTGATGCTGAATGCTGGGCGCGTGGTGAAATGACTATCGCTGCCGCCGTGGACCAGTACA
AAGCGCGTGTTCGGCTCCAGATGGCATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7