Detailed information of TA system TA188152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4101955..4102649
• Replicon: chromosome
• Accession: NZ_CP068810
• Organism: Escherichia coli strain RIVM_C028536

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: JNN60_RS19740
• Protein ID: WP_001263491.1
• Coordinates: 4101955..4102353 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: JNN60_RS19745
• Protein ID: WP_000554755.1
• Coordinates: 4102356..4102649 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN60_RS19710	4097124..4098368	-	1245	WP_000189541.1	esterase FrsA	-
-	4097784..4097864	-	81	NuclAT_10	-	-
-	4097784..4097864	-	81	NuclAT_10	-	-
-	4097784..4097864	-	81	NuclAT_10	-	-
-	4097784..4097864	-	81	NuclAT_10	-	-
JNN60_RS19715	4098460..4098918	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JNN60_RS19720	4099179..4100636	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JNN60_RS19725	4100693..4101045	-	353	Protein_3866	peptide chain release factor H	-
JNN60_RS19730	4101041..4101247	-	207	Protein_3867	RtcB family protein	-
JNN60_RS19735	4101493..4101945	-	453	WP_019842500.1	GNAT family N-acetyltransferase	-
JNN60_RS19740	4101955..4102353	-	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN60_RS19745	4102356..4102649	-	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN60_RS19750	4102701..4103756	-	1056	WP_001550655.1	DNA polymerase IV	-
JNN60_RS19755	4103827..4104612	-	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
JNN60_RS19760	4104584..4106296	+	1713	Protein_3873	flagellar biosynthesis protein FlhA	-
JNN60_RS19765	4106401..4106679	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
JNN60_RS19770	4106672..4107028	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4091891..4102649	10758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T188152 WP_001263491.1 NZ_CP068810:c4102353-4101955 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T188152 NZ_CP090312:577644-577994 [Bacillus licheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGATTCCGTTATATTGCTCGAACAAATT
CGGACGATTGATAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT188152 WP_000554755.1 NZ_CP068810:c4102649-4102356 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT188152 NZ_CP090312:577358-577639 [Bacillus licheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTGCGCGAGCGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATCAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5