Detailed information of TA system TA188129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4871703..4872305
• Replicon: chromosome
• Accession: NZ_CP068809
• Organism: Escherichia coli strain RIVM_C028497

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN39_RS23240
• Protein ID: WP_000897305.1
• Coordinates: 4871994..4872305 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN39_RS23235
• Protein ID: WP_000356397.1
• Coordinates: 4871703..4871993 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN39_RS23215	4868205..4869107	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN39_RS23220	4869104..4869739	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN39_RS23225	4869736..4870665	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN39_RS23230	4870880..4871098	-	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
JNN39_RS23235	4871703..4871993	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
JNN39_RS23240	4871994..4872305	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN39_RS23245	4872534..4873442	+	909	WP_001298596.1	alpha/beta hydrolase	-
JNN39_RS23250	4873506..4874447	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN39_RS23255	4874492..4874929	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JNN39_RS23260	4874926..4875798	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JNN39_RS23265	4875792..4876391	-	600	WP_001298594.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T188129 WP_000897305.1 NZ_CP068809:c4872305-4871994 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T188129 NZ_CP090304:4241649-4241960 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT188129 WP_000356397.1 NZ_CP068809:c4871993-4871703 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT188129 NZ_CP090304:4241961-4242251 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7