Detailed information of TA system TA188119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3925071..3925765
• Replicon: chromosome
• Accession: NZ_CP068809
• Organism: Escherichia coli strain RIVM_C028497

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: JNN39_RS18705
• Protein ID: WP_001263500.1
• Coordinates: 3925071..3925469 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: JNN39_RS18710
• Protein ID: WP_000554758.1
• Coordinates: 3925472..3925765 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN39_RS18680	3920436..3920894	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
JNN39_RS18685	3921155..3922612	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
JNN39_RS18690	3922669..3923283	-	615	WP_000602124.1	peptide chain release factor H	-
JNN39_RS18695	3923280..3924419	-	1140	WP_000521561.1	RNA ligase RtcB family protein	-
JNN39_RS18700	3924609..3925061	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
JNN39_RS18705	3925071..3925469	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN39_RS18710	3925472..3925765	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN39_RS18715	3925817..3926872	-	1056	WP_001226168.1	DNA polymerase IV	-
JNN39_RS18720	3926943..3927728	-	786	WP_000207578.1	putative lateral flagellar export/assembly protein LafU	-
JNN39_RS18725	3927700..3929412	+	1713	Protein_3671	flagellar biosynthesis protein FlhA	-
JNN39_RS18730	3929732..3930229	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	blaOXA-48	gmhA/lpcA	3925071..3967297	42226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T188119 WP_001263500.1 NZ_CP068809:c3925469-3925071 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T188119 NZ_CP090304:c528352-528134 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT188119 WP_000554758.1 NZ_CP068809:c3925765-3925472 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT188119 NZ_CP090304:c528754-528380 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3