Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1929689..1930191 Replicon chromosome
Accession NZ_CP068809
Organism Escherichia coli strain RIVM_C028497

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag JNN39_RS09035 Protein ID WP_000767822.1
Coordinates 1929937..1930191 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JNN39_RS09030 Protein ID WP_001259255.1
Coordinates 1929689..1929940 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN39_RS09005 1924867..1925934 - 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
JNN39_RS09010 1925934..1927004 - 1071 WP_000108995.1 histidinol-phosphate transaminase -
JNN39_RS09015 1927001..1928305 - 1305 WP_001297912.1 histidinol dehydrogenase -
JNN39_RS09020 1928311..1929210 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
JNN39_RS09025 1929356..1929406 - 51 WP_001364200.1 his operon leader peptide -
JNN39_RS09030 1929689..1929940 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JNN39_RS09035 1929937..1930191 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JNN39_RS09040 1930274..1931098 + 825 WP_202847282.1 SDR family oxidoreductase -
JNN39_RS09045 1931144..1932073 + 930 WP_000803366.1 LysR family transcriptional regulator -
JNN39_RS09050 1932288..1932350 + 63 WP_010723108.1 membrane protein YoeI -
JNN39_RS09055 1932340..1933698 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T188102 WP_000767822.1 NZ_CP068809:1929937-1930191 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T188102 NZ_CP090244:c1475005-1474700 [Serratia marcescens]
ATGCCCGCGTTTCGCCTCACCCCGCAGGCCCAACAAGATCTGCTGGCTATTCGTCATTTCACCATCGAGCACTGGGGTCA
AGCGCAATCTCGCCGCTATCTTGAGCAGTTGAGAGAGGTCATGCATCATTTGGCAGATATGCCGGAAGCGGGTAAAGCTC
ACTTCCACGATCTGGGCGAAGAGATACACAGTTTTCCTTACGCCAGCCATAGGATCTACTACCGCAACCGCCCTGCCGGC
ATCACCGTTCTCACCATTCTGCATCAGGCGATGGTGCCTCACCGGCATCTGGAACAACGGCTGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT188102 WP_001259255.1 NZ_CP068809:1929689-1929940 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT188102 NZ_CP090244:c1475267-1475007 [Serratia marcescens]
ATGTACGCACTCACCGCGAACGAGGCCAAAACCCAATTCGGCGATTTGCTGCTCAAGGCGCAGCGTGAACCGGTGCAAAT
CAATCGCAACGGCAAACCGGTCGCCGTCGTCATGTCTGTAGAAGATTACCAGGCGCTGGAAGATCTCAAATTAAGCGCAC
TGCAGCAAAAAATCGCGCAGGCTACCGCCGAGGAAGACGCCGGTAAGGCGGTAGACGGCGATGCCTTCTTCAACCGTCTG
CTCACCGATGAAACAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References