Detailed information of TA system TA188095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5091515..5092117
• Replicon: chromosome
• Accession: NZ_CP068808
• Organism: Escherichia coli strain RIVM_C018699

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN47_RS24675
• Protein ID: WP_000897305.1
• Coordinates: 5091806..5092117 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN47_RS24670
• Protein ID: WP_000356395.1
• Coordinates: 5091515..5091805 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN47_RS24635	5087139..5088041	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN47_RS24640	5088038..5088673	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN47_RS24645	5088670..5089599	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN47_RS24650	5089781..5090023	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
JNN47_RS24655	5090242..5090460	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN47_RS24660	5090879..5091157	-	279	WP_001315112.1	hypothetical protein	-
JNN47_RS24665	5091209..5091430	-	222	WP_001550354.1	hypothetical protein	-
JNN47_RS24670	5091515..5091805	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
JNN47_RS24675	5091806..5092117	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN47_RS24680	5092346..5093254	+	909	WP_001550353.1	alpha/beta hydrolase	-
JNN47_RS24685	5093422..5095236	-	1815	WP_001550352.1	hypothetical protein	-
JNN47_RS24690	5095652..5096593	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN47_RS24695	5096638..5097075	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T188095 WP_000897305.1 NZ_CP068808:c5092117-5091806 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T188095 NZ_CP090242:4083006-4083374 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACTGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT188095 WP_000356395.1 NZ_CP068808:c5091805-5091515 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT188095 NZ_CP090242:4082788-4083009 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7