Detailed information of TA system TA188088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4091924..4092618
• Replicon: chromosome
• Accession: NZ_CP068808
• Organism: Escherichia coli strain RIVM_C018699

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: JNN47_RS19845
• Protein ID: WP_001263491.1
• Coordinates: 4091924..4092322 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: JNN47_RS19850
• Protein ID: WP_000554755.1
• Coordinates: 4092325..4092618 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN47_RS19815	4087093..4088337	-	1245	WP_000189541.1	esterase FrsA	-
-	4087753..4087833	-	81	NuclAT_10	-	-
-	4087753..4087833	-	81	NuclAT_10	-	-
-	4087753..4087833	-	81	NuclAT_10	-	-
-	4087753..4087833	-	81	NuclAT_10	-	-
JNN47_RS19820	4088429..4088887	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JNN47_RS19825	4089148..4090605	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JNN47_RS19830	4090662..4091014	-	353	Protein_3883	peptide chain release factor H	-
JNN47_RS19835	4091010..4091216	-	207	Protein_3884	RtcB family protein	-
JNN47_RS19840	4091462..4091914	-	453	WP_019842500.1	GNAT family N-acetyltransferase	-
JNN47_RS19845	4091924..4092322	-	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN47_RS19850	4092325..4092618	-	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN47_RS19855	4092670..4093725	-	1056	WP_001550655.1	DNA polymerase IV	-
JNN47_RS19860	4093796..4094581	-	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
JNN47_RS19865	4094553..4096265	+	1713	Protein_3890	flagellar biosynthesis protein FlhA	-
JNN47_RS19870	4096370..4096648	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
JNN47_RS19875	4096641..4096997	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4081860..4092618	10758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T188088 WP_001263491.1 NZ_CP068808:c4092322-4091924 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T188088 NZ_CP090222:c5081155-5080871 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACCATTTAACCAAACACAGAAATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT188088 WP_000554755.1 NZ_CP068808:c4092618-4092325 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT188088 NZ_CP090222:c5081393-5081145 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGTTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5