Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2012468..2012970 Replicon chromosome
Accession NZ_CP068808
Organism Escherichia coli strain RIVM_C018699

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JNN47_RS09675 Protein ID WP_000767829.1
Coordinates 2012716..2012970 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JNN47_RS09670 Protein ID WP_001259255.1
Coordinates 2012468..2012719 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN47_RS09645 2007646..2008713 - 1068 WP_000080135.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
JNN47_RS09650 2008713..2009783 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
JNN47_RS09655 2009786..2011084 - 1299 WP_000009595.1 histidinol dehydrogenase -
JNN47_RS09660 2011090..2011989 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
JNN47_RS09665 2012135..2012185 - 51 WP_001364200.1 his operon leader peptide -
JNN47_RS09670 2012468..2012719 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JNN47_RS09675 2012716..2012970 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JNN47_RS09680 2013053..2013877 + 825 WP_000754737.1 SDR family oxidoreductase -
JNN47_RS09685 2013923..2014852 + 930 Protein_1895 LysR family transcriptional regulator -
JNN47_RS09690 2015067..2015129 + 63 WP_010723108.1 membrane protein YoeI -
JNN47_RS09695 2015119..2016477 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JNN47_RS09700 2016656..2017714 + 1059 WP_000492339.1 transport protein YeeE -
JNN47_RS09705 2017728..2017955 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T188072 WP_000767829.1 NZ_CP068808:2012716-2012970 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T188072 NZ_CP090218:c4621992-4621708 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCTGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT188072 WP_001259255.1 NZ_CP068808:2012468-2012719 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT188072 NZ_CP090218:c4622224-4621982 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References