Detailed information of TA system TA188071

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1210581..1211308
• Replicon: chromosome
• Accession: NZ_CP068808
• Organism: Escherichia coli strain RIVM_C018699

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: JNN47_RS05830
• Protein ID: WP_000547555.1
• Coordinates: 1210581..1210892 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN47_RS05835
• Protein ID: WP_000126294.1
• Coordinates: 1210889..1211308 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN47_RS05805	1206516..1208225	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
JNN47_RS05810	1208235..1208777	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
JNN47_RS05815	1208777..1209544	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
JNN47_RS05820	1209541..1209951	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
JNN47_RS05825	1209944..1210414	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
JNN47_RS05830	1210581..1210892	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
JNN47_RS05835	1210889..1211308	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
JNN47_RS05840	1211387..1212811	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
JNN47_RS05845	1212820..1214277	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
JNN47_RS05850	1214537..1215547	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
JNN47_RS05855	1215696..1216223	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T188071 WP_000547555.1 NZ_CP068808:1210581-1210892 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T188071 NZ_CP090218:c4521541-4521008 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT188071 WP_000126294.1 NZ_CP068808:1210889-1211308 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT188071 NZ_CP090218:c4521818-4521552 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: V0YLE2