Detailed information of TA system TA188059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4157557..4158251
• Replicon: chromosome
• Accession: NZ_CP068807
• Organism: Escherichia coli strain RIVM_C018583

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: JNN51_RS19940
• Protein ID: WP_001263491.1
• Coordinates: 4157853..4158251 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: JNN51_RS19935
• Protein ID: WP_000554755.1
• Coordinates: 4157557..4157850 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN51_RS19910	4153178..4153534	-	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-
JNN51_RS19915	4153527..4153805	-	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
JNN51_RS19920	4153910..4155622	-	1713	Protein_3885	flagellar biosynthesis protein FlhA	-
JNN51_RS19925	4155594..4156379	+	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
JNN51_RS19930	4156450..4157505	+	1056	WP_001550655.1	DNA polymerase IV	-
JNN51_RS19935	4157557..4157850	+	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN51_RS19940	4157853..4158251	+	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN51_RS19945	4158261..4158713	+	453	WP_019842500.1	GNAT family N-acetyltransferase	-
JNN51_RS19950	4158959..4159165	+	207	Protein_3891	RtcB family protein	-
JNN51_RS19955	4159161..4159513	+	353	Protein_3892	peptide chain release factor H	-
JNN51_RS19960	4159570..4161027	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JNN51_RS19965	4161288..4161746	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JNN51_RS19970	4161838..4163082	+	1245	WP_000189541.1	esterase FrsA	-
-	4162342..4162422	+	81	NuclAT_11	-	-
-	4162342..4162422	+	81	NuclAT_11	-	-
-	4162342..4162422	+	81	NuclAT_11	-	-
-	4162342..4162422	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T188059 WP_001263491.1 NZ_CP068807:4157853-4158251 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T188059 NZ_CP090213:84760-85176 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT188059 WP_000554755.1 NZ_CP068807:4157557-4157850 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT188059 NZ_CP090213:84533-84763 [Escherichia coli]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5