Detailed information of TA system TA188049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2276271..2277070
• Replicon: chromosome
• Accession: NZ_CP068807
• Organism: Escherichia coli strain RIVM_C018583

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F0JQM9
• Locus tag: JNN51_RS10945
• Protein ID: WP_000347270.1
• Coordinates: 2276606..2277070 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A0V9H1L4
• Locus tag: JNN51_RS10940
• Protein ID: WP_001551693.1
• Coordinates: 2276271..2276606 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN51_RS10925	2272056..2272826	-	771	WP_001551692.1	2-dehydro-3-deoxyglucarate aldolase	-
JNN51_RS10930	2272842..2274176	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
JNN51_RS10935	2274551..2276122	+	1572	WP_033878411.1	galactarate dehydratase	-
JNN51_RS10940	2276271..2276606	+	336	WP_001551693.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JNN51_RS10945	2276606..2277070	+	465	WP_000347270.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JNN51_RS10950	2277125..2277934	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JNN51_RS10955	2278183..2279463	+	1281	WP_001551694.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JNN51_RS10960	2279486..2279959	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JNN51_RS10965	2279970..2280749	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
JNN51_RS10970	2280739..2281617	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
JNN51_RS10975	2281635..2282069	+	435	WP_000948837.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2267509..2277070	9561

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17806.22 Da
• Isoelectric Point: 9.6924

>T188049 WP_000347270.1 NZ_CP068807:2276606-2277070 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Nucleotide

• Length: 465 bp

>T188049 NZ_CP090212:922622-923029 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12347.87 Da
• Isoelectric Point: 4.6890

>AT188049 WP_001551693.1 NZ_CP068807:2276271-2276606 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT188049 NZ_CP090212:922375-922641 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0V9NQ90

Antitoxin

• AlphaFold DB: A0A0V9H1L4