Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4223336..4224135 Replicon chromosome
Accession NZ_CP068806
Organism Escherichia coli strain RIVM_C018576

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag JNN57_RS20275 Protein ID WP_000347269.1
Coordinates 4223671..4224135 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0YUB5
Locus tag JNN57_RS20270 Protein ID WP_001309780.1
Coordinates 4223336..4223671 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN57_RS20255 4219121..4219891 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
JNN57_RS20260 4219907..4221241 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
JNN57_RS20265 4221616..4223187 + 1572 WP_001273738.1 galactarate dehydratase -
JNN57_RS20270 4223336..4223671 + 336 WP_001309780.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JNN57_RS20275 4223671..4224135 + 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JNN57_RS20280 4224190..4224999 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
JNN57_RS20285 4225248..4226528 + 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
JNN57_RS20290 4226551..4227024 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JNN57_RS20295 4227035..4227814 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JNN57_RS20300 4227804..4228682 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JNN57_RS20305 4228700..4229134 + 435 WP_000948818.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4214094..4224135 10041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T188027 WP_000347269.1 NZ_CP068806:4223671-4224135 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T188027 NZ_CP090203:4527512-4527880 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12345.94 Da        Isoelectric Point: 4.8616

>AT188027 WP_001309780.1 NZ_CP068806:4223336-4223671 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT188027 NZ_CP090203:4527294-4527515 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB V0YUB5

References