Detailed information of TA system TA188003

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 638432..638690
• Replicon: chromosome
• Accession: NZ_CP068806
• Organism: Escherichia coli strain RIVM_C018576

Toxin (Protein)

• Gene name: hokC
• Uniprot ID: S1NX00
• Locus tag: JNN57_RS03025
• Protein ID: WP_000809168.1
• Coordinates: 638432..638584 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	sokC
Locus tag	-
Coordinates	638633..638690 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN57_RS03005	633674..634387	-	714	WP_001102367.1	acidic protein MsyB	-
JNN57_RS03010	634413..634817	-	405	WP_000843584.1	DUF2541 family protein	-
JNN57_RS03015	635193..637109	+	1917	WP_000516135.1	molecular chaperone DnaK	-
JNN57_RS03020	637198..638328	+	1131	WP_001118464.1	molecular chaperone DnaJ	-
JNN57_RS03025	638432..638584	-	153	WP_000809168.1	type I toxin-antitoxin system toxin MokC	Toxin
-	638633..638690	+	58	-	-	Antitoxin
JNN57_RS03030	639170..640336	+	1167	WP_000681360.1	Na+/H+ antiporter NhaA	-
JNN57_RS03035	640402..641301	+	900	WP_001300855.1	transcriptional activator NhaR	-
JNN57_RS03040	641339..642298	-	960	WP_000871674.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5501.57 Da
• Isoelectric Point: 7.1312

>T188003 WP_000809168.1 NZ_CP068806:c638584-638432 [Escherichia coli]
MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVFTAYESE

Nucleotide

• Length: 153 bp

>T188003 NZ_CP090196:c1974678-1974304 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Nucleotide

• Length: 58 bp

>AT188003 NZ_CP068806:638633-638690 [Escherichia coli]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Structures

Toxin

• AlphaFold DB: S1NX00

Antitoxin