Detailed information of TA system TA187999

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 546525..546937
• Replicon: chromosome
• Accession: NZ_CP068806
• Organism: Escherichia coli strain RIVM_C018576

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: JNN57_RS02575
• Protein ID: WP_000132601.1
• Coordinates: 546525..546866 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	546861..546937 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN57_RS02565	543938..544984	-	1047	WP_147697023.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-
JNN57_RS02570	544984..546363	-	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
JNN57_RS02575	546525..546866	-	342	WP_000132601.1	endoribonuclease SymE	Toxin
-	546861..546937	+	77	NuclAT_12	-	Antitoxin
-	546861..546937	+	77	NuclAT_12	-	Antitoxin
-	546861..546937	+	77	NuclAT_12	-	Antitoxin
-	546861..546937	+	77	NuclAT_12	-	Antitoxin
-	546861..546937	+	77	NuclAT_13	-	Antitoxin
-	546861..546937	+	77	NuclAT_13	-	Antitoxin
-	546861..546937	+	77	NuclAT_13	-	Antitoxin
-	546861..546937	+	77	NuclAT_13	-	Antitoxin
JNN57_RS02580	547094..548488	-	1395	WP_001272445.1	type I restriction-modification system specificity subunit	-
JNN57_RS02585	548485..550074	-	1590	WP_001063200.1	type I restriction-modification system methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	534234..548488	14254

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T187999 WP_000132601.1 NZ_CP068806:c546866-546525 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T187999 NZ_CP090196:1152376-1152711 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Nucleotide

• Length: 77 bp

>AT187999 NZ_CP068806:546861-546937 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin