Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 5012559..5013161 Replicon chromosome
Accession NZ_CP068805
Organism Escherichia coli strain RIVM_C018567

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag JNN55_RS24250 Protein ID WP_000897305.1
Coordinates 5012850..5013161 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JNN55_RS24245 Protein ID WP_000356395.1
Coordinates 5012559..5012849 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN55_RS24210 5008183..5009085 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
JNN55_RS24215 5009082..5009717 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
JNN55_RS24220 5009714..5010643 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
JNN55_RS24225 5010825..5011067 - 243 WP_001309881.1 ribbon-helix-helix domain-containing protein -
JNN55_RS24230 5011286..5011504 - 219 WP_001295676.1 ribbon-helix-helix domain-containing protein -
JNN55_RS24235 5011923..5012201 - 279 WP_001315112.1 hypothetical protein -
JNN55_RS24240 5012253..5012474 - 222 WP_001550354.1 hypothetical protein -
JNN55_RS24245 5012559..5012849 - 291 WP_000356395.1 helix-turn-helix domain-containing protein Antitoxin
JNN55_RS24250 5012850..5013161 - 312 WP_000897305.1 hypothetical protein Toxin
JNN55_RS24255 5013390..5014298 + 909 WP_001550353.1 alpha/beta hydrolase -
JNN55_RS24260 5014466..5016280 - 1815 WP_001550352.1 hypothetical protein -
JNN55_RS24265 5016696..5017637 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
JNN55_RS24270 5017682..5018119 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T187996 WP_000897305.1 NZ_CP068805:c5013161-5012850 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T187996 NZ_CP090196:c839878-838985 [Escherichia coli]
ATGCCTGAATTAACATCATTATTTCCTGACGTTGCCGATGCACTGGATATTGAATCTGTCGCTATCGTCGAAAAAGATTA
TTTTGTGGTTGATCTTTTAAGATTATTAAAAGAAATAAAACCAGAAACACATACTCTCGTGTTTGCAGGTAGTACCGCAT
TATCAAAAGCAGGTATCTCGCTAAACAGAATGTCAGAAGATATTGATATAAAGCTCGTGCCAACAGAGAACTTTATGCAA
AATGGCAGAGACAAGAGAAGAAAAATTCGCAAGGAAATCGTGCAAATAATCACGGATGTTATTCATAATTCTGACATTTT
CAGCTTGGATAATGAAAACGCCCGTATAACACGTGATGAGTATCGTTATAACGAAATCTCGGTACGTTATCCCCAGACAT
TTGCACAAGTCCCCTGTCTTCGTCCATTTATTAAGCTGGAGTTGATGGAAAGCACTCTGCTGGAACATCCGGAAAGCAGA
GATATTTATTCCCTTGTAACGGAGTTAACGGGTAAAGGTACACCTATAACAGCATTCCCCTGTGCCACCATTTTATCCAC
TCAGGCTGAAAAGCTCATCTCCATGATGCGAAGAACAGCAGCACATCTTCGTAATCCCGAACAACAGGATGATGAGTTTC
TGGTACGTCACATTTATGACAACTACTGCATTGTCAGGGAAAAAGGTGTGAATGTTCCTGTATTGAAAAATTTTGTTCAG
ATATGTATTCAACAGGATATTGAACGCTATGGTAACCAGTACCCACAATTTTGTAGCTCACCAGTTGATGAACTTAAGAA
AGGGCTGGATGCACTCCGGGATTATCCTGTACACAAATTGCGCTTTCAACGATTTGTTAACCCGATGGTATTTGGTAATA
CACAGATTAACTAG

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT187996 WP_000356395.1 NZ_CP068805:c5012849-5012559 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT187996 NZ_CP090196:c840266-839871 [Escherichia coli]
ATGACTACAAAAGACCGTATTCAGTCCCGACTGAACCGTTCAAAACGTTATGTGTTCACCCGGGATGATTTCAGGGATAT
CGCTGGCTATGATCAGGTTGGCAGAGCTCTTCGTACACTGGTAAACGAAGGTAAGTTGATGAAAGTGGGCTATGGTGTTT
ACACCAAAGCCCGTCGTAACATCATCACTGGGAAAATCATGCCCGCAGCGCCTGGGGGCTCTGCTGCAGTAATTACAGAG
GCCCTGGAGCGCCTGAATATCAGATACCGTCTGGCAGGAGCAACGGAATGCTACAATAAAGGTCAGTCCACACAAATTCC
TGTATCTCTGGGGATAAAAACACCTTCTCGATTTAAAAGAGTGTTAATAGTAGGGAAAAGTAAAATCAATGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References