Detailed information of TA system TA187996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5012559..5013161
• Replicon: chromosome
• Accession: NZ_CP068805
• Organism: Escherichia coli strain RIVM_C018567

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN55_RS24250
• Protein ID: WP_000897305.1
• Coordinates: 5012850..5013161 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN55_RS24245
• Protein ID: WP_000356395.1
• Coordinates: 5012559..5012849 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN55_RS24210	5008183..5009085	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN55_RS24215	5009082..5009717	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN55_RS24220	5009714..5010643	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN55_RS24225	5010825..5011067	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
JNN55_RS24230	5011286..5011504	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN55_RS24235	5011923..5012201	-	279	WP_001315112.1	hypothetical protein	-
JNN55_RS24240	5012253..5012474	-	222	WP_001550354.1	hypothetical protein	-
JNN55_RS24245	5012559..5012849	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
JNN55_RS24250	5012850..5013161	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN55_RS24255	5013390..5014298	+	909	WP_001550353.1	alpha/beta hydrolase	-
JNN55_RS24260	5014466..5016280	-	1815	WP_001550352.1	hypothetical protein	-
JNN55_RS24265	5016696..5017637	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN55_RS24270	5017682..5018119	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T187996 WP_000897305.1 NZ_CP068805:c5013161-5012850 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T187996 NZ_CP090196:c839878-838985 [Escherichia coli]
ATGCCTGAATTAACATCATTATTTCCTGACGTTGCCGATGCACTGGATATTGAATCTGTCGCTATCGTCGAAAAAGATTA
TTTTGTGGTTGATCTTTTAAGATTATTAAAAGAAATAAAACCAGAAACACATACTCTCGTGTTTGCAGGTAGTACCGCAT
TATCAAAAGCAGGTATCTCGCTAAACAGAATGTCAGAAGATATTGATATAAAGCTCGTGCCAACAGAGAACTTTATGCAA
AATGGCAGAGACAAGAGAAGAAAAATTCGCAAGGAAATCGTGCAAATAATCACGGATGTTATTCATAATTCTGACATTTT
CAGCTTGGATAATGAAAACGCCCGTATAACACGTGATGAGTATCGTTATAACGAAATCTCGGTACGTTATCCCCAGACAT
TTGCACAAGTCCCCTGTCTTCGTCCATTTATTAAGCTGGAGTTGATGGAAAGCACTCTGCTGGAACATCCGGAAAGCAGA
GATATTTATTCCCTTGTAACGGAGTTAACGGGTAAAGGTACACCTATAACAGCATTCCCCTGTGCCACCATTTTATCCAC
TCAGGCTGAAAAGCTCATCTCCATGATGCGAAGAACAGCAGCACATCTTCGTAATCCCGAACAACAGGATGATGAGTTTC
TGGTACGTCACATTTATGACAACTACTGCATTGTCAGGGAAAAAGGTGTGAATGTTCCTGTATTGAAAAATTTTGTTCAG
ATATGTATTCAACAGGATATTGAACGCTATGGTAACCAGTACCCACAATTTTGTAGCTCACCAGTTGATGAACTTAAGAA
AGGGCTGGATGCACTCCGGGATTATCCTGTACACAAATTGCGCTTTCAACGATTTGTTAACCCGATGGTATTTGGTAATA
CACAGATTAACTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT187996 WP_000356395.1 NZ_CP068805:c5012849-5012559 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT187996 NZ_CP090196:c840266-839871 [Escherichia coli]
ATGACTACAAAAGACCGTATTCAGTCCCGACTGAACCGTTCAAAACGTTATGTGTTCACCCGGGATGATTTCAGGGATAT
CGCTGGCTATGATCAGGTTGGCAGAGCTCTTCGTACACTGGTAAACGAAGGTAAGTTGATGAAAGTGGGCTATGGTGTTT
ACACCAAAGCCCGTCGTAACATCATCACTGGGAAAATCATGCCCGCAGCGCCTGGGGGCTCTGCTGCAGTAATTACAGAG
GCCCTGGAGCGCCTGAATATCAGATACCGTCTGGCAGGAGCAACGGAATGCTACAATAAAGGTCAGTCCACACAAATTCC
TGTATCTCTGGGGATAAAAACACCTTCTCGATTTAAAAGAGTGTTAATAGTAGGGAAAAGTAAAATCAATGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7