Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4070282..4070976 Replicon chromosome
Accession NZ_CP068805
Organism Escherichia coli strain RIVM_C018567

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag JNN55_RS19710 Protein ID WP_001263491.1
Coordinates 4070282..4070680 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag JNN55_RS19715 Protein ID WP_000554755.1
Coordinates 4070683..4070976 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN55_RS19680 4065451..4066695 - 1245 WP_000189541.1 esterase FrsA -
- 4066111..4066191 - 81 NuclAT_10 - -
- 4066111..4066191 - 81 NuclAT_10 - -
- 4066111..4066191 - 81 NuclAT_10 - -
- 4066111..4066191 - 81 NuclAT_10 - -
JNN55_RS19685 4066787..4067245 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
JNN55_RS19690 4067506..4068963 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
JNN55_RS19695 4069020..4069372 - 353 Protein_3858 peptide chain release factor H -
JNN55_RS19700 4069368..4069574 - 207 Protein_3859 RtcB family protein -
JNN55_RS19705 4069820..4070272 - 453 WP_019842500.1 GNAT family N-acetyltransferase -
JNN55_RS19710 4070282..4070680 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JNN55_RS19715 4070683..4070976 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JNN55_RS19720 4071028..4072083 - 1056 WP_001550655.1 DNA polymerase IV -
JNN55_RS19725 4072154..4072939 - 786 WP_019842510.1 putative lateral flagellar export/assembly protein LafU -
JNN55_RS19730 4072911..4074623 + 1713 Protein_3865 flagellar biosynthesis protein FlhA -
JNN55_RS19735 4074728..4075006 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JNN55_RS19740 4074999..4075355 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4060218..4070976 10758


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T187989 WP_001263491.1 NZ_CP068805:c4070680-4070282 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T187989 NZ_CP090192:c3994189-3993698 [Enterobacter hormaechei]
ATGATCTCTGCCCCAGTGCCCCTTCGAGCCGGGCATATTCTTACTCCATTTTGCTGTGGGGTAGATTCGATGGATAACTG
GCTTAAGCAGCGGGCGATGAAAAATCAGGCCAGTGGTGCATCGCGTACATTTGTCTGTTGTGGCGGAGATTCTAACGTAC
TGGCTTATTACGCGCTGGCGTCCAGCGCGGTCACAACGAATACGGTACCCGGCCGCTTTCGTCGCAATATGCCCGATCCC
ATCCCTGTTGTAGTGCTGGGACGCCTGGCGGTAGATCAGTCTCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCCGGGCTGCGGGTGATCCAGGTGGCGGAGAGCATCGGCATCCGCGGAATGCTGGTTCATGCCCTGTCGGATGAAGCGC
GGGAATTTTATCTGCGGGTGGGGTTTGAGCCATCGCCGATGGATCCGATGATATTGATGGTGACGTTGGGGGATTTGGTG
GCGAGTATTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT187989 WP_000554755.1 NZ_CP068805:c4070976-4070683 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT187989 NZ_CP090192:c3994479-3994186 [Enterobacter hormaechei]
ATGCCAGCTGCAAACACTATGGCGGTGAAACGCGAAACCCTTAACCTGCGCATCAGGCCTGCCGAGCGGGATCTTATCGA
TCGGGCAGCGAAAGCGAGAGGGAAAAACCGTACTGACTTTGTGCTGGAAGCAGCCCGCGCAGCTGCGGAAGAGGCACTGA
TCGAACAACGCATCATAATGGCCGATCCAGAGGCTTATCAGGAATTTCTGACCCGTCTGGATCAGGCTCCAGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCTTGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References