Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1990030..1990532 Replicon chromosome
Accession NZ_CP068805
Organism Escherichia coli strain RIVM_C018567

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JNN55_RS09530 Protein ID WP_000767829.1
Coordinates 1990278..1990532 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JNN55_RS09525 Protein ID WP_001259255.1
Coordinates 1990030..1990281 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN55_RS09500 1985208..1986275 - 1068 WP_000080135.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
JNN55_RS09505 1986275..1987345 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
JNN55_RS09510 1987348..1988646 - 1299 WP_000009595.1 histidinol dehydrogenase -
JNN55_RS09515 1988652..1989551 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
JNN55_RS09520 1989697..1989747 - 51 WP_001364200.1 his operon leader peptide -
JNN55_RS09525 1990030..1990281 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JNN55_RS09530 1990278..1990532 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JNN55_RS09535 1990615..1991439 + 825 WP_000754737.1 SDR family oxidoreductase -
JNN55_RS09540 1991485..1992414 + 930 Protein_1868 LysR family transcriptional regulator -
JNN55_RS09545 1992629..1992691 + 63 WP_010723108.1 membrane protein YoeI -
JNN55_RS09550 1992681..1994039 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JNN55_RS09555 1994218..1995276 + 1059 WP_000492339.1 transport protein YeeE -
JNN55_RS09560 1995290..1995517 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T187973 WP_000767829.1 NZ_CP068805:1990278-1990532 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T187973 NZ_CP090179:560289-560576 [Vibrio harveyi]
ATGAAAGTAGTCTGGTCTCCATTAGCACTTCAGAAACTAGGTGATGCAGCGGAGTTTATTTCTTTGGATAATCCACCTGC
AGCAGAGAGGTGGGTAAACGAAGTTTTTGATAAAACTGAACTACTTGGAAGTATGCCTGAAATGGGGCGGTTTGTTCCTG
AAATGCCCCACACGAACTATCGCGAAATCATCTTTGGTCACTATCGTATTATCTACAGTTTAAGTCACGAAATCCGCGTA
CTAACAGTTCGTAATTGTCGTCAAATTTTATCGGAAGATGACGTGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT187973 WP_001259255.1 NZ_CP068805:1990030-1990281 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT187973 NZ_CP090179:560011-560292 [Vibrio harveyi]
ATGAGTCGTATTCATTTCGATCAAGATATTCAGCCTTTGTCTGAGTTTCGTGCCGGTGTAGCTTCATACATCAAACAAAT
CAATGAGACTCGTCGTCCATTGGTTATTACGCAACGTGGCAAAGGTGTTGCCGTTGTTCTCGATGTTGCTGAGTATGAAG
CAATGCAAGAGAAAATCGAATTACTGGAAGAAATGCGCACAGCAGAAGCTCAGTTAGCTTCTGGTCTCGGTGTTTCCAAT
GATGATGCGCGTGCTCAAGTACTTGGGCGCATTAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References