Detailed information of TA system TA187966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4977399..4978001
• Replicon: chromosome
• Accession: NZ_CP068804
• Organism: Escherichia coli strain RIVM_C018563

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN37_RS23890
• Protein ID: WP_000897305.1
• Coordinates: 4977690..4978001 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN37_RS23885
• Protein ID: WP_000356395.1
• Coordinates: 4977399..4977689 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN37_RS23850	4973023..4973925	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN37_RS23855	4973922..4974557	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN37_RS23860	4974554..4975483	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN37_RS23865	4975665..4975907	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
JNN37_RS23870	4976126..4976344	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN37_RS23875	4976763..4977041	-	279	WP_001315112.1	hypothetical protein	-
JNN37_RS23880	4977093..4977314	-	222	WP_001550354.1	hypothetical protein	-
JNN37_RS23885	4977399..4977689	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
JNN37_RS23890	4977690..4978001	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN37_RS23895	4978230..4979138	+	909	WP_001550353.1	alpha/beta hydrolase	-
JNN37_RS23900	4979306..4981120	-	1815	WP_001550352.1	hypothetical protein	-
JNN37_RS23905	4981536..4982477	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN37_RS23910	4982522..4982959	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T187966 WP_000897305.1 NZ_CP068804:c4978001-4977690 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T187966 NZ_CP090146:c3994236-3993745 [Enterobacter hormaechei]
ATGATCTCTGCCCCAGTGCCCCTTCGAGCCGGGCATATTCTTACTCCATTTTGCTGTGGGGTAGATTCGATGGATAACTG
GCTTAAGCAGCGGGCGATGAAAAATCAGGCCAGTGGTGCATCGCGTACATTTGTCTGTTGTGGCGGAGATTCTAACGTAC
TGGCTTATTACGCGCTGGCGTCCAGCGCGGTCACAACGAATACGGTACCCGGCCGCTTTCGTCGCAATATGCCCGATCCC
ATCCCTGTTGTAGTGCTGGGACGCCTGGCGGTAGATCAGTCTCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCCGGGCTGCGGGTGATCCAGGTGGCGGAGAGCATCGGCATCCGCGGAATGCTGGTTCATGCCCTGTCGGATGAAGCGC
GGGAATTTTATCTGCGGGTGGGGTTTGAGCCATCGCCGATGGATCCGATGATATTGATGGTGACGTTGGGGGATTTGGTG
GCGAGTATTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT187966 WP_000356395.1 NZ_CP068804:c4977689-4977399 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT187966 NZ_CP090146:c3994526-3994233 [Enterobacter hormaechei]
ATGCCAGCTGCAAACACTATGGCGGTGAAACGCGAAACCCTTAACCTGCGCATCAGGCCTGCCGAGCGGGATCTTATCGA
TCGGGCAGCGAAAGCGAGAGGGAAAAACCGTACTGACTTTGTGCTGGAAGCAGCCCGCGCAGCTGCGGAAGAGGCACTGA
TCGAACAACGCATCATAATGGCCGATCCAGAGGCTTATCAGGAATTTCTGACCCGTCTGGATCAGGCTCCAGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCTTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7