Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4020545..4021239 Replicon chromosome
Accession NZ_CP068804
Organism Escherichia coli strain RIVM_C018563

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag JNN37_RS19280 Protein ID WP_001263491.1
Coordinates 4020545..4020943 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag JNN37_RS19285 Protein ID WP_000554755.1
Coordinates 4020946..4021239 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN37_RS19250 4015714..4016958 - 1245 WP_000189541.1 esterase FrsA -
- 4016374..4016454 - 81 NuclAT_10 - -
- 4016374..4016454 - 81 NuclAT_10 - -
- 4016374..4016454 - 81 NuclAT_10 - -
- 4016374..4016454 - 81 NuclAT_10 - -
JNN37_RS19255 4017050..4017508 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
JNN37_RS19260 4017769..4019226 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
JNN37_RS19265 4019283..4019635 - 353 Protein_3775 peptide chain release factor H -
JNN37_RS19270 4019631..4019837 - 207 Protein_3776 RtcB family protein -
JNN37_RS19275 4020083..4020535 - 453 WP_019842500.1 GNAT family N-acetyltransferase -
JNN37_RS19280 4020545..4020943 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JNN37_RS19285 4020946..4021239 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JNN37_RS19290 4021291..4022346 - 1056 WP_001550655.1 DNA polymerase IV -
JNN37_RS19295 4022417..4023202 - 786 WP_019842510.1 putative lateral flagellar export/assembly protein LafU -
JNN37_RS19300 4023174..4024886 + 1713 Protein_3782 flagellar biosynthesis protein FlhA -
JNN37_RS19305 4024991..4025269 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JNN37_RS19310 4025262..4025618 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4010481..4021239 10758


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T187960 WP_001263491.1 NZ_CP068804:c4020943-4020545 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T187960 NZ_CP090146:30480-30794 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAATACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT187960 WP_000554755.1 NZ_CP068804:c4021239-4020946 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT187960 NZ_CP090146:30794-31210 [Enterobacter hormaechei]
ATGATCGTCGCTGATGCAATGAAAGCTACGCATGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGAGAA
AGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTTTGCG
CCAGAATTAGCCATTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGAATT
GCAGTTTTGAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGATGGT
TTCACGGGTTCTGAATGGTCAGAGACAGCTTACTCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCACCTG
CGCTATTTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References