Detailed information of TA system TA187945

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1232597..1233324
• Replicon: chromosome
• Accession: NZ_CP068804
• Organism: Escherichia coli strain RIVM_C018563

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: JNN37_RS05960
• Protein ID: WP_000547555.1
• Coordinates: 1232597..1232908 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN37_RS05965
• Protein ID: WP_000126294.1
• Coordinates: 1232905..1233324 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN37_RS05935	1228532..1230241	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
JNN37_RS05940	1230251..1230793	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
JNN37_RS05945	1230793..1231560	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
JNN37_RS05950	1231557..1231967	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
JNN37_RS05955	1231960..1232430	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
JNN37_RS05960	1232597..1232908	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
JNN37_RS05965	1232905..1233324	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
JNN37_RS05970	1233403..1234827	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
JNN37_RS05975	1234836..1236293	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
JNN37_RS05980	1236553..1237563	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
JNN37_RS05985	1237712..1238239	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T187945 WP_000547555.1 NZ_CP068804:1232597-1232908 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T187945 NZ_CP090137:c4000474-4000190 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGACGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGAAGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT187945 WP_000126294.1 NZ_CP068804:1232905-1233324 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT187945 NZ_CP090137:c4000706-4000464 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: V0YLE2