Detailed information of TA system TA187940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 629163..629962
• Replicon: chromosome
• Accession: NZ_CP068804
• Organism: Escherichia coli strain RIVM_C018563

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F0JQM9
• Locus tag: JNN37_RS02980
• Protein ID: WP_000347270.1
• Coordinates: 629163..629627 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A0V9H1L4
• Locus tag: JNN37_RS02985
• Protein ID: WP_001551693.1
• Coordinates: 629627..629962 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN37_RS02950	624164..624598	-	435	WP_000948837.1	PTS sugar transporter subunit IIA	-
JNN37_RS02955	624616..625494	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
JNN37_RS02960	625484..626263	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
JNN37_RS02965	626274..626747	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JNN37_RS02970	626770..628050	-	1281	WP_001551694.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JNN37_RS02975	628299..629108	+	810	WP_106106567.1	aga operon transcriptional regulator AgaR	-
JNN37_RS02980	629163..629627	-	465	WP_000347270.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JNN37_RS02985	629627..629962	-	336	WP_001551693.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JNN37_RS02990	630111..631682	-	1572	WP_001273738.1	galactarate dehydratase	-
JNN37_RS02995	632057..633391	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
JNN37_RS03000	633407..634177	+	771	WP_001551692.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17806.22 Da
• Isoelectric Point: 9.6924

>T187940 WP_000347270.1 NZ_CP068804:c629627-629163 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Nucleotide

• Length: 465 bp

>T187940 NZ_CP090137:842690-843103 [Salmonella enterica subsp. enterica serovar Goldcoast]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CGCGCCAACACTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12347.87 Da
• Isoelectric Point: 4.6890

>AT187940 WP_001551693.1 NZ_CP068804:c629962-629627 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT187940 NZ_CP090137:842443-842709 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0V9NQ90

Antitoxin

• AlphaFold DB: A0A0V9H1L4