Detailed information of TA system TA187938

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4619834..4620436
• Replicon: chromosome
• Accession: NZ_CP068803
• Organism: Escherichia coli strain RIVM_C018404

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN34_RS22295
• Protein ID: WP_000897305.1
• Coordinates: 4620125..4620436 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN34_RS22290
• Protein ID: WP_000356397.1
• Coordinates: 4619834..4620124 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN34_RS22265	4615760..4616662	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN34_RS22270	4616659..4617294	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN34_RS22275	4617291..4618220	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
JNN34_RS22280	4618550..4618792	-	243	WP_001086388.1	hypothetical protein	-
JNN34_RS22285	4619011..4619229	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN34_RS22290	4619834..4620124	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
JNN34_RS22295	4620125..4620436	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN34_RS22300	4620665..4621573	+	909	WP_001162704.1	alpha/beta hydrolase	-
JNN34_RS22305	4621637..4622578	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN34_RS22310	4622623..4623060	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JNN34_RS22315	4623057..4623929	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JNN34_RS22320	4623923..4624522	-	600	WP_086732335.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T187938 WP_000897305.1 NZ_CP068803:c4620436-4620125 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T187938 NZ_CP090137:213667-214152 [Salmonella enterica subsp. enterica serovar Goldcoast]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGGGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT187938 WP_000356397.1 NZ_CP068803:c4620124-4619834 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT187938 NZ_CP090137:213392-213679 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGCTATACAAAGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCGGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATTAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7