Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 620579..621378 Replicon chromosome
Accession NZ_CP068803
Organism Escherichia coli strain RIVM_C018404

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag JNN34_RS02980 Protein ID WP_000347266.1
Coordinates 620579..621043 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JNN34_RS02985 Protein ID WP_001307405.1
Coordinates 621043..621378 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN34_RS02950 615580..616014 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
JNN34_RS02955 616032..616910 - 879 WP_001315856.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JNN34_RS02960 616900..617679 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JNN34_RS02965 617690..618163 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JNN34_RS02970 618186..619466 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
JNN34_RS02975 619715..620524 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JNN34_RS02980 620579..621043 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JNN34_RS02985 621043..621378 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JNN34_RS02990 621527..623098 - 1572 WP_001273741.1 galactarate dehydratase -
JNN34_RS02995 623473..624807 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JNN34_RS03000 624823..625593 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 620579..632348 11769


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T187915 WP_000347266.1 NZ_CP068803:c621043-620579 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T187915 NZ_CP090126:c3962164-3961847 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT187915 WP_001307405.1 NZ_CP068803:c621378-621043 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT187915 NZ_CP090126:c3961854-3961567 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References