Detailed information of TA system TA187903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2109692..2110386
• Replicon: chromosome
• Accession: NZ_CP068802
• Organism: Escherichia coli strain RIVM_C018249

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A1X0YXS1
• Locus tag: JNN40_RS10260
• Protein ID: WP_001263484.1
• Coordinates: 2109692..2110090 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: JNN40_RS10265
• Protein ID: WP_000554755.1
• Coordinates: 2110093..2110386 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN40_RS10230	2104862..2106106	-	1245	WP_000189541.1	esterase FrsA	-
-	2105522..2105602	-	81	NuclAT_8	-	-
-	2105522..2105602	-	81	NuclAT_8	-	-
-	2105522..2105602	-	81	NuclAT_8	-	-
-	2105522..2105602	-	81	NuclAT_8	-	-
JNN40_RS10235	2106198..2106656	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JNN40_RS10240	2106917..2108374	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JNN40_RS10245	2108431..2108783	-	353	Protein_2008	peptide chain release factor H	-
JNN40_RS10250	2108779..2108985	-	207	Protein_2009	RtcB family protein	-
JNN40_RS10255	2109230..2109682	-	453	WP_001059899.1	GNAT family N-acetyltransferase	-
JNN40_RS10260	2109692..2110090	-	399	WP_001263484.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN40_RS10265	2110093..2110386	-	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN40_RS10270	2110438..2111493	-	1056	WP_001226183.1	DNA polymerase IV	-
JNN40_RS10275	2111564..2112349	-	786	WP_000207554.1	putative lateral flagellar export/assembly protein LafU	-
JNN40_RS10280	2112321..2114033	+	1713	Protein_2015	flagellar biosynthesis protein FlhA	-
JNN40_RS10285	2114138..2114416	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
JNN40_RS10290	2114409..2114765	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15404.81 Da
• Isoelectric Point: 7.3840

>T187903 WP_001263484.1 NZ_CP068802:c2110090-2109692 [Escherichia coli]
MRVFKTKLICLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T187903 NZ_CP090103:141267-141542 [Rhizobium leguminosarum]
ATGGAACTCAAATGGACGAGCAAAGCGGTCTCCGACGTTGCCCGCTTGTACGACTTTCTCGCTCCTGTAGACCGCCTCGC
GGCGGCTCGAACCGTCCAGGCACTGACGAAGGCACCTGCGCGGCTTCTTGATCAGCCGCGCCTCGGCGAGCGGCTTGAGG
AGTTCGATCCTCGGGAGGTCCGCCGCATTCTCATCGGCCGTTACGAGCTGCGCTATGAAATCCAGCAGTCGACGATCTAT
GTGTTGCGGCTTTGGCATACGCGTGAGGACCGATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT187903 WP_000554755.1 NZ_CP068802:c2110386-2110093 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT187903 NZ_CP090103:141007-141267 [Rhizobium leguminosarum]
ATGGAGACCAAGGTTCTGACCGCCCATGTTCCGCTGTCGCTTGCGCAGAAGGTCGATCAACTCGCGTCGCGGCTCGAACG
CTCGCGTGGCTGGATCGTCAAGCAGGCCCTGACGGCATGGATCGACCAGGAGGAGGAGCGCCGGCGTTTGACGCTTGAGG
CGTTGGCCGACGTCGATGACGGTAACGTTGTCGATCACCAGTCTGTCCAAGCCTGGGCCGATAGTCTTGACGGCGACGCG
CCGATTTCGTTGCCTCGGTGA

Structures

Toxin

• AlphaFold DB: A0A1X0YXS1

Antitoxin

• AlphaFold DB: A0A829G9H5