Detailed information of TA system TA187889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5140543..5141145
• Replicon: chromosome
• Accession: NZ_CP068801
• Organism: Escherichia coli strain RIVM_C018220

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN52_RS24980
• Protein ID: WP_000897305.1
• Coordinates: 5140834..5141145 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN52_RS24975
• Protein ID: WP_000356395.1
• Coordinates: 5140543..5140833 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN52_RS24940	5136167..5137069	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN52_RS24945	5137066..5137701	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN52_RS24950	5137698..5138627	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN52_RS24955	5138809..5139051	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
JNN52_RS24960	5139270..5139488	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN52_RS24965	5139907..5140185	-	279	WP_001315112.1	hypothetical protein	-
JNN52_RS24970	5140237..5140458	-	222	WP_001550354.1	hypothetical protein	-
JNN52_RS24975	5140543..5140833	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
JNN52_RS24980	5140834..5141145	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN52_RS24985	5141374..5142282	+	909	WP_001550353.1	alpha/beta hydrolase	-
JNN52_RS24990	5142450..5144264	-	1815	WP_001550352.1	hypothetical protein	-
JNN52_RS24995	5144680..5145621	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN52_RS25000	5145666..5146103	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T187889 WP_000897305.1 NZ_CP068801:c5141145-5140834 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T187889 NZ_CP090095:c1402009-1401710 [Neorhizobium galegae]
ATGACGGTTGAGACAAAGAAATGGGATATCACCGAGCATCTGGACACCGATGAGCGTATCGCGCTCTTTCTGGAAGCGGT
GTTTGAAGATGGCGATCCGGCCGTGATCGCCGCGGCGATCGGCGATGTCGCCCGAGCCCGAGGCATGAGCCAGATCGCCA
AGGATGCCGGCCTCTCACGTGAAAATCTCTATCGAGCATTGAGTGAGGGCGGCAATCCCGAGTTCGCGACCATCCTGAAG
GTCATCCGAGCCATCGGCTACGATCTCACCATTGTACCGCATGGCTCCGCAGCTCGCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT187889 WP_000356395.1 NZ_CP068801:c5140833-5140543 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT187889 NZ_CP090095:c1402308-1402006 [Neorhizobium galegae]
ATGATCGAAATCCAGGAAACCGAAATCTATTCGACTTGGTTTGCCAGGTTGCGGGACGAGCGGGCCAAAGCCCGGATCAA
TGCCCGCATCTATCGTTTGTCGCTCGGCAATCCCGGCGATGTAAAGCCTGTCGGTTCCGGTGTCAGCGAAATGCGGGTCG
ATTACGGACCGGGTTTTCGGATCTATTTCATCCGCCGTGGCCAGGCATTCGTCGTCCTGCTCTGCGGCGGAGACAAATCT
AGCCAGACGGAAGATATCGTATCGGCCAAAAAGCTGGCGGCTGGTCTGGAGGGCCGGAAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7