Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 4090758..4091452 | Replicon | chromosome |
| Accession | NZ_CP068801 | ||
| Organism | Escherichia coli strain RIVM_C018220 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | Q0T7Q5 |
| Locus tag | JNN52_RS19830 | Protein ID | WP_001263491.1 |
| Coordinates | 4090758..4091156 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | L4JHF0 |
| Locus tag | JNN52_RS19835 | Protein ID | WP_000554755.1 |
| Coordinates | 4091159..4091452 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JNN52_RS19800 | 4085927..4087171 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| - | 4086587..4086667 | - | 81 | NuclAT_11 | - | - |
| - | 4086587..4086667 | - | 81 | NuclAT_11 | - | - |
| - | 4086587..4086667 | - | 81 | NuclAT_11 | - | - |
| - | 4086587..4086667 | - | 81 | NuclAT_11 | - | - |
| JNN52_RS19805 | 4087263..4087721 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| JNN52_RS19810 | 4087982..4089439 | + | 1458 | WP_001293009.1 | cytosol nonspecific dipeptidase | - |
| JNN52_RS19815 | 4089496..4089848 | - | 353 | Protein_3882 | peptide chain release factor H | - |
| JNN52_RS19820 | 4089844..4090050 | - | 207 | Protein_3883 | RtcB family protein | - |
| JNN52_RS19825 | 4090296..4090748 | - | 453 | WP_019842500.1 | GNAT family N-acetyltransferase | - |
| JNN52_RS19830 | 4090758..4091156 | - | 399 | WP_001263491.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| JNN52_RS19835 | 4091159..4091452 | - | 294 | WP_000554755.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| JNN52_RS19840 | 4091504..4092559 | - | 1056 | WP_001550655.1 | DNA polymerase IV | - |
| JNN52_RS19845 | 4092630..4093415 | - | 786 | WP_019842510.1 | putative lateral flagellar export/assembly protein LafU | - |
| JNN52_RS19850 | 4093387..4095099 | + | 1713 | Protein_3889 | flagellar biosynthesis protein FlhA | - |
| JNN52_RS19855 | 4095204..4095482 | + | 279 | WP_000598760.1 | type II toxin-antitoxin system HicA family toxin | - |
| JNN52_RS19860 | 4095475..4095831 | + | 357 | WP_001030484.1 | type II toxin-antitoxin system HicB family antitoxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 4080694..4091452 | 10758 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15457.86 Da Isoelectric Point: 8.0949
>T187882 WP_001263491.1 NZ_CP068801:c4091156-4090758 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T187882 NZ_CP090093:942436-942933 [Rhizobium leguminosarum]
GTGACCGTAAGCGTACCCGCGCCGCTTGCTGATCATCACGAGCTAGCCGAATTCCATTCTGGTGTCCCTGAGCTTGATGA
TTGGCTGCGTCTCCGCGCCCGCGCCAATCAGGCGAGCGGCGCATCGCGTACCTTCGTCGTGTGTGAGGCCAACCGCGTCA
TCGCCTACTACGCGCTCGCTTCCGGCGCGGTAAAGCAATCGGAAGCATCGGGACGTTTCCGGCGGAACATGCCTGATCCG
ATACCAGTCGCCGTGCTTGGACGTCTTGCCATTGATCAATCCTATCAGGGACGTGGTTTCGGCAGGGCGTTAGTGCGCGA
CGCAGGTCTGCGCTTGATCAATGCCGCCGAGATACTTGGGATTCGCGGCGTTTTAGTCCATGCGATCTCGGATGACGCAC
GAGCCTTTTACGAAGCGGTTGGCTTCCTGCCTTCCCCGTCCGACCCGATGATGCTGCTGGTCGGGTTGCATGACCTCAAC
AATGCGCTGACCTCCTGA
GTGACCGTAAGCGTACCCGCGCCGCTTGCTGATCATCACGAGCTAGCCGAATTCCATTCTGGTGTCCCTGAGCTTGATGA
TTGGCTGCGTCTCCGCGCCCGCGCCAATCAGGCGAGCGGCGCATCGCGTACCTTCGTCGTGTGTGAGGCCAACCGCGTCA
TCGCCTACTACGCGCTCGCTTCCGGCGCGGTAAAGCAATCGGAAGCATCGGGACGTTTCCGGCGGAACATGCCTGATCCG
ATACCAGTCGCCGTGCTTGGACGTCTTGCCATTGATCAATCCTATCAGGGACGTGGTTTCGGCAGGGCGTTAGTGCGCGA
CGCAGGTCTGCGCTTGATCAATGCCGCCGAGATACTTGGGATTCGCGGCGTTTTAGTCCATGCGATCTCGGATGACGCAC
GAGCCTTTTACGAAGCGGTTGGCTTCCTGCCTTCCCCGTCCGACCCGATGATGCTGCTGGTCGGGTTGCATGACCTCAAC
AATGCGCTGACCTCCTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11235.78 Da Isoelectric Point: 5.2170
>AT187882 WP_000554755.1 NZ_CP068801:c4091452-4091159 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT187882 NZ_CP090093:942143-942439 [Rhizobium leguminosarum]
ATGCCGACCTCACACACGAGCAAAAGCCCGGACGCAAGCGTGCCGCTGAATATGCGCATCAAGCCTGCGACGCGCAACTT
GATTGACCGCGCCGCTGAGATGCTAGGCAAGACACGTACAGATTTCATGCTGGAGGCTTCCGAGCGCCGTGCCGAGGAAG
TACTACTTGACCGGACGATCTTCACTGTCAGCGCCGAAGTCTACGCAGAGTATCTGGCCCGGCTCGATGCGCCAACACAG
CCCAATGATCGTCTTAAACGCACTATGTCGACCAAAGCGCCGTGGGATGAGGCGTGA
ATGCCGACCTCACACACGAGCAAAAGCCCGGACGCAAGCGTGCCGCTGAATATGCGCATCAAGCCTGCGACGCGCAACTT
GATTGACCGCGCCGCTGAGATGCTAGGCAAGACACGTACAGATTTCATGCTGGAGGCTTCCGAGCGCCGTGCCGAGGAAG
TACTACTTGACCGGACGATCTTCACTGTCAGCGCCGAAGTCTACGCAGAGTATCTGGCCCGGCTCGATGCGCCAACACAG
CCCAATGATCGTCTTAAACGCACTATGTCGACCAAAGCGCCGTGGGATGAGGCGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4P7TPK7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829G9H5 |