187865

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2043052..2043554 Replicon chromosome
Accession NZ_CP068801
Organism Escherichia coli strain RIVM_C018220

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JNN52_RS09850 Protein ID WP_000767829.1
Coordinates 2043300..2043554 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JNN52_RS09845 Protein ID WP_001259255.1
Coordinates 2043052..2043303 (+) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN52_RS09820 2038230..2039297 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
JNN52_RS09825 2039297..2040367 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
JNN52_RS09830 2040364..2041668 - 1305 WP_039267630.1 histidinol dehydrogenase -
JNN52_RS09835 2041674..2042573 - 900 WP_000131789.1 ATP phosphoribosyltransferase -
JNN52_RS09840 2042719..2042769 - 51 WP_071593552.1 his operon leader peptide -
JNN52_RS09845 2043052..2043303 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JNN52_RS09850 2043300..2043554 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JNN52_RS09855 2043637..2044461 + 825 WP_000754737.1 SDR family oxidoreductase -
JNN52_RS09860 2044507..2045436 + 930 WP_000803351.1 LysR family transcriptional regulator -
JNN52_RS09865 2045651..2045713 + 63 WP_010723108.1 membrane protein YoeI -
JNN52_RS09870 2045703..2047061 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JNN52_RS09875 2047240..2048298 + 1059 WP_023568741.1 transport protein YeeE -
JNN52_RS09880 2048312..2048539 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-84)

Antitoxin

(1-73)


Sequences


Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T187865 WP_000767829.1 NZ_CP068801:2043300-2043554 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T187865 NZ_CP090068:c47475-47188 [Acinetobacter sp. SH20PTE14]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATGTCTCTTTCGAAACTGCGAG
CCTTGTTTTTGAAGATCCACTACGGATCTCAATCCAGGACAGACATACCGATGGCGAAGAACGTTGGCAGACCATTGGAA
AAGTAAAAGGCGTACTAATGCTCTTAGTAGCTCATACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAGGTAACTAAAGCGGAGCGAGATAAATATGAGCATGGTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT187865 WP_001259255.1 NZ_CP068801:2043052-2043303 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT187865 NZ_CP090068:c47201-46887 [Acinetobacter sp. SH20PTE14]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAAAAATTCAGCGACAAGCAAGATGCTGAAATTGAACGCTTGCT
TGCTAAAGGTATAGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATGACTGATTGGAGTAATGCTGCACGCC
ATAATCAATTCTATCGCCCTGTGAAGCAACAAACTTCTATTCGGTTAGATGCTGATGTGCTTGCTTGGTTCAAAGCACAA
GGTAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTTAAAGAATTAAAAAATCATCAATAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q


Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References