Detailed information of TA system TA187864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1240097..1240824
• Replicon: chromosome
• Accession: NZ_CP068801
• Organism: Escherichia coli strain RIVM_C018220

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: JNN52_RS06000
• Protein ID: WP_000547555.1
• Coordinates: 1240097..1240408 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN52_RS06005
• Protein ID: WP_000126294.1
• Coordinates: 1240405..1240824 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN52_RS05975	1236032..1237741	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
JNN52_RS05980	1237751..1238293	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
JNN52_RS05985	1238293..1239060	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
JNN52_RS05990	1239057..1239467	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
JNN52_RS05995	1239460..1239930	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
JNN52_RS06000	1240097..1240408	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
JNN52_RS06005	1240405..1240824	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
JNN52_RS06010	1240903..1242327	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
JNN52_RS06015	1242336..1243793	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
JNN52_RS06020	1244053..1245063	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
JNN52_RS06025	1245212..1245739	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T187864 WP_000547555.1 NZ_CP068801:1240097-1240408 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T187864 NZ_CP090067:c3250995-3250693 [Acinetobacter sp. SH20PTE14]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAACAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAACCAGTTGGTGAAGGTGTCTCTGAATTACGTTTCT
TCTTTGGACCAGGCTATAGGATTTACTACTGCAAACAAGGGAAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT187864 WP_000126294.1 NZ_CP068801:1240405-1240824 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT187864 NZ_CP090067:c3250692-3250411 [Acinetobacter sp. SH20PTE14]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAAGAAGATATGCAGGCTTACTTACAAGCATG
TATCGAAGAATCTAATGGTGATGCCGCATTCATTGCTAAAGCTTTAGGTAACATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGTCTAGGCCGAGAAAGTCTTTACAAAGCACTTTCTGGTGATGTGAACCCTAGTTTTGATACGGTG
ATTAAAGTTGTAAAAGCACTAAATTTGCGCTTAGTAATTTAA

Structures

Toxin

• AlphaFold DB: V0YLE2