Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 670889..671688 Replicon chromosome
Accession NZ_CP068801
Organism Escherichia coli strain RIVM_C018220

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag JNN52_RS03215 Protein ID WP_000347270.1
Coordinates 670889..671353 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag JNN52_RS03220 Protein ID WP_001551693.1
Coordinates 671353..671688 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN52_RS03185 665890..666324 - 435 WP_202847431.1 PTS sugar transporter subunit IIA -
JNN52_RS03190 666342..667220 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JNN52_RS03195 667210..667989 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JNN52_RS03200 668000..668473 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JNN52_RS03205 668496..669776 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
JNN52_RS03210 670025..670834 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JNN52_RS03215 670889..671353 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JNN52_RS03220 671353..671688 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JNN52_RS03225 671837..673408 - 1572 WP_001273738.1 galactarate dehydratase -
JNN52_RS03230 673783..675117 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
JNN52_RS03235 675133..675903 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T187859 WP_000347270.1 NZ_CP068801:c671353-670889 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

>T187859 NZ_CP090066:c2832056-2831790 [Aurantimonas sp. HBX-1]
ATGAAGCTTCTCTGGGCCGAGCAGGCCTGGAGCGACTATCTGCATTTTCAGAAGGTGGACGAGAAGATCCGCGATCGCAT
CAACGAGCTGATCAAGGAAACCAGGCGAACCCTGCTCCGGGGGATCGGCAAGCCGGAGCCGCTGAAGGGCGATCTGACCG
GCTGGTGGTCGCGGCGGATCACCGGCGACCACCGCCTTGTCTGCCGCGTTGCAGGCAAAGATGACGACCAACGCCTCGAA
ATCGCGGCGTGCCGCTATAAGCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT187859 WP_001551693.1 NZ_CP068801:c671688-671353 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT187859 NZ_CP090066:c2832319-2832053 [Aurantimonas sp. HBX-1]
ATGGCGGACCGCTTTCACGCAGTTGCGGGCTATCCAGCCGGCCACTTCGAAGAGTTGATCCGCTCGCGGGCGCCCTTGCT
CGTGACCCGGCAGGGCAGAGAATCCGCCGTATTGATATCGGCGAGCGAATGGGAGGGCGTCCAGGAGACGCTTCACCTTC
TCGGCAGCCGGAACAACGCCGACAAGCTGTTCGGCGCCATTGCCGAACTGGATGCCGGTCGCGGTGTCGAGCGCGATCCG
ACCGTCGACGCCGAAGACGCCCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References