Detailed information of TA system TA187856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4971404..4972006
• Replicon: chromosome
• Accession: NZ_CP068800
• Organism: Escherichia coli strain RIVM_C017997

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN45_RS23765
• Protein ID: WP_000897305.1
• Coordinates: 4971695..4972006 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN45_RS23760
• Protein ID: WP_000356395.1
• Coordinates: 4971404..4971694 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN45_RS23725	4967028..4967930	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN45_RS23730	4967927..4968562	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN45_RS23735	4968559..4969488	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN45_RS23740	4969670..4969912	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
JNN45_RS23745	4970131..4970349	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN45_RS23750	4970768..4971046	-	279	WP_001315112.1	hypothetical protein	-
JNN45_RS23755	4971098..4971319	-	222	WP_001550354.1	hypothetical protein	-
JNN45_RS23760	4971404..4971694	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
JNN45_RS23765	4971695..4972006	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN45_RS23770	4972235..4973143	+	909	WP_001550353.1	alpha/beta hydrolase	-
JNN45_RS23775	4973311..4975125	-	1815	WP_001550352.1	hypothetical protein	-
JNN45_RS23780	4975541..4976482	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN45_RS23785	4976527..4976964	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T187856 WP_000897305.1 NZ_CP068800:c4972006-4971695 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T187856 NZ_CP090065:c2977169-2976849 [Pectobacterium polonicum]
ATGCAAACAACACCTGCAATCCCGCCACGGGAGGATAACCCCTGTCCGTCACCTGTAGCCGTCTGGCAGCAACTTCTGAC
CTACCTGCTGGAAAAGCACTACGGCCTCACGCTGAACGATACGCCGCTCTGTGAGGAAAATGTGATTCAGGAGCATATCG
ATGCTGGCGTTACACTGGTCAATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGATCGCAACGGCTTT
AACTGGCAGGAGCAATCGCCATTTCTTACTGCCGTTGATGTTCTCCGCGCCAGACGTGTTACCGGCTTGCTCAAGGCATA
A

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT187856 WP_000356395.1 NZ_CP068800:c4971694-4971404 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT187856 NZ_CP090065:c2977574-2977239 [Pectobacterium polonicum]
ATGCCATCATCTGATAGCCCGGAATGGGGGCTAAAATGTGCCATCACTCCGCGATTCGGAGCCAGACTGGTGCAGGAAGG
TAACCGTCTGCATTATCTGGTAGATCGGGCCAACATTGTCGGTAAGTTCAGCAAAACAGAAGCCCAAAGTCTGGAACGTT
GCTTCCCTGAGCTGATTAAGCTGTTGGAGCAGAAGCTACGCACGGGTGAACTGAATCCCCGCCAGCAGGACTGCATCATT
CTGCATTGCGATGAATGTACCTGTGAAGCCGATACGCTGGGCAGTTTTGGCTACGTGTATATTGTTATTTATCCCTCTTC
TGCGGCAGCAGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7