Detailed information of TA system TA187850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4074657..4075351
• Replicon: chromosome
• Accession: NZ_CP068800
• Organism: Escherichia coli strain RIVM_C017997

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: JNN45_RS19545
• Protein ID: WP_001263491.1
• Coordinates: 4074657..4075055 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: JNN45_RS19550
• Protein ID: WP_000554755.1
• Coordinates: 4075058..4075351 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN45_RS19515	4069826..4071070	-	1245	WP_000189541.1	esterase FrsA	-
-	4070486..4070566	-	81	NuclAT_10	-	-
-	4070486..4070566	-	81	NuclAT_10	-	-
-	4070486..4070566	-	81	NuclAT_10	-	-
-	4070486..4070566	-	81	NuclAT_10	-	-
JNN45_RS19520	4071162..4071620	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JNN45_RS19525	4071881..4073338	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JNN45_RS19530	4073395..4073747	-	353	Protein_3827	peptide chain release factor H	-
JNN45_RS19535	4073743..4073949	-	207	Protein_3828	RtcB family protein	-
JNN45_RS19540	4074195..4074647	-	453	WP_019842500.1	GNAT family N-acetyltransferase	-
JNN45_RS19545	4074657..4075055	-	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN45_RS19550	4075058..4075351	-	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN45_RS19555	4075403..4076458	-	1056	WP_001550655.1	DNA polymerase IV	-
JNN45_RS19560	4076529..4077314	-	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
JNN45_RS19565	4077286..4078998	+	1713	Protein_3834	flagellar biosynthesis protein FlhA	-
JNN45_RS19570	4079103..4079381	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
JNN45_RS19575	4079374..4079730	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4064593..4075351	10758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T187850 WP_001263491.1 NZ_CP068800:c4075055-4074657 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T187850 NZ_CP090065:c645028-644723 [Pectobacterium polonicum]
ATGCAATTCATTGTTTATCAATACAAACAAGCCAGTCACTACAAAATGTTTGTCGATGTACAAAGTGATATTGTCGAGAC
ACTAAAACGATGTATGGCTATTCCACTTATCGAATCGCATTACCTGTCAGAGAAAGTGAATAAGGTGTTGTTCCCGCTGA
TCCGCATCGATGGCGAAGATTATCGGCTGATGACGACTGAGCTATCGAGCGTTCCCGTTGAGGTCATTGGTGAAGTGATT
GCGGATCTTGGCGACTACGCCGATGAGGTTAAGGATGCTCTTAATCTTATGTTTTGGGGGATTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT187850 WP_000554755.1 NZ_CP068800:c4075351-4075058 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT187850 NZ_CP090065:c645249-645031 [Pectobacterium polonicum]
ATGAAACACCGCGTCAGCGTTACCGTAGACAAAGACAATTATCAGGTTCTGAGTGCTGCCGGAATCAATATTTCTGGGCT
GGTGAATGACGCCATTGGCAAAGAAGCCCGCCGTATCAGAACTGAGATGTGGAAGAAGGAAAAAAGCGAAGGGATGGAAG
ACGTAGCCAGGTTTATAGCCCAGCATGATTCTTTTGCTGATGAAAACAGAAACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5