Detailed information of TA system TA187835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1278618..1279345
• Replicon: chromosome
• Accession: NZ_CP068800
• Organism: Escherichia coli strain RIVM_C017997

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: JNN45_RS06180
• Protein ID: WP_000547555.1
• Coordinates: 1278618..1278929 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN45_RS06185
• Protein ID: WP_000126294.1
• Coordinates: 1278926..1279345 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN45_RS06155	1274553..1276262	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
JNN45_RS06160	1276272..1276814	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
JNN45_RS06165	1276814..1277581	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
JNN45_RS06170	1277578..1277988	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
JNN45_RS06175	1277981..1278451	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
JNN45_RS06180	1278618..1278929	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
JNN45_RS06185	1278926..1279345	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
JNN45_RS06190	1279424..1280848	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
JNN45_RS06195	1280857..1282314	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
JNN45_RS06200	1282574..1283584	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
JNN45_RS06205	1283733..1284260	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T187835 WP_000547555.1 NZ_CP068800:1278618-1278929 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T187835 NZ_CP090061:c3257627-3257244 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT187835 WP_000126294.1 NZ_CP068800:1278926-1279345 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT187835 NZ_CP090061:c3257869-3257627 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: V0YLE2