Detailed information of TA system TA187827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5013327..5013929
• Replicon: chromosome
• Accession: NZ_CP068799
• Organism: Escherichia coli strain RIVM_C017887

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN46_RS23965
• Protein ID: WP_000897305.1
• Coordinates: 5013618..5013929 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN46_RS23960
• Protein ID: WP_000356397.1
• Coordinates: 5013327..5013617 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN46_RS23940	5009829..5010731	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN46_RS23945	5010728..5011363	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN46_RS23950	5011360..5012289	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN46_RS23955	5012504..5012722	-	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
JNN46_RS23960	5013327..5013617	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
JNN46_RS23965	5013618..5013929	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN46_RS23970	5014158..5015066	+	909	WP_001298596.1	alpha/beta hydrolase	-
JNN46_RS23975	5015130..5016071	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN46_RS23980	5016116..5016553	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JNN46_RS23985	5016550..5017422	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JNN46_RS23990	5017416..5018015	-	600	WP_001298594.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T187827 WP_000897305.1 NZ_CP068799:c5013929-5013618 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T187827 NZ_CP090059:3278612-3279004 [Sphingomonas cannabina]
ATGATCGTCGACGCCTCCGTCCTCGTGGCGCTCCTCCGCGAAGAGCCCGAAACCGCATGGATCGAGCCGATATTGAACGC
GAACTATGGAACGCTGAAAATGTCGGTCGCGAACTATCTCGAAGCGGCGATCGTCATCGATGCCAATGACGACGAGGTTC
TCAGCGAACGGCTGGAACTGGTCATCGCCCATTTCGGGATCGAGCTGATACCGATGAACGCCTATCATGCGCGCATCGGT
CGGGAAGCCTTCAACAGATACGGTAAGGGTCGTCATCGCGCCCGTCTCAATTATGGGGACTGTTTCGCCTACGCTCTGGC
CAGAGGCACCAACGAACCCCTTCTTTTCAAGGGCGACGACTTCAACCACACCGACATTACCTTGGCGCGGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT187827 WP_000356397.1 NZ_CP068799:c5013617-5013327 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT187827 NZ_CP090059:3278337-3278615 [Sphingomonas cannabina]
ATGGGCGTTCAGCTCAATATCAAGAGCGCGGAAGCAAGGGCACTCGCCGAAGAACTCGCCGAAGCCACGGGGGAAAGCAT
TACCGAGGCGATCACCGCCGCTCTCCGCCTCAGGCTGCGGCAGATCGCGCTCGAGAAGGCGAAGGAGCCGAATGCCATGC
GTGAGCGCGAAATGGCCTTCTATCGCCTTATCCGGGGCAGCCGGATGCGTTGGAAGGGTGCGATGCTTTCCGTCGATCAT
GGCGATCTGCTTTATGACGAGCACGGATTGCCGAGATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7