Detailed information of TA system TA187821

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4404485..4404897
• Replicon: chromosome
• Accession: NZ_CP068799
• Organism: Escherichia coli strain RIVM_C017887

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: JNN46_RS21160
• Protein ID: WP_000132614.1
• Coordinates: 4404556..4404897 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4404485..4404561 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN46_RS21150	4401160..4402629	+	1470	WP_001540814.1	type I restriction-modification system subunit M	-
JNN46_RS21155	4402629..4404335	+	1707	WP_001100194.1	restriction endonuclease subunit S	-
-	4404485..4404561	-	77	NuclAT_6	-	Antitoxin
-	4404485..4404561	-	77	NuclAT_6	-	Antitoxin
-	4404485..4404561	-	77	NuclAT_6	-	Antitoxin
-	4404485..4404561	-	77	NuclAT_6	-	Antitoxin
-	4404485..4404561	-	77	NuclAT_7	-	Antitoxin
-	4404485..4404561	-	77	NuclAT_7	-	Antitoxin
-	4404485..4404561	-	77	NuclAT_7	-	Antitoxin
-	4404485..4404561	-	77	NuclAT_7	-	Antitoxin
JNN46_RS21160	4404556..4404897	+	342	WP_000132614.1	endoribonuclease SymE	Toxin
JNN46_RS21165	4404944..4407037	-	2094	WP_000648237.1	DUF262 domain-containing protein	-
JNN46_RS21170	4407135..4407215	-	81	WP_020233658.1	hypothetical protein	-
JNN46_RS21175	4407443..4408363	-	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
JNN46_RS21180	4408548..4409828	+	1281	WP_001298033.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	4388364..4407100	18736

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T187821 WP_000132614.1 NZ_CP068799:4404556-4404897 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T187821 NZ_CP090028:c4720052-4719777 [Serratia ureilytica]
ATGGAGTTAAAATGGACGAGTAAGGTGCTACCGGATTTGGCGCGTTTGTTTGAGTTTCTTGCCTTGGCTAACCGCACTGC
GGCAGCGAATTCGGTGAAGGCCTTGGTCGCCGCGCCTAAAGCGTTGCTCGATAATCCTCGCCTGGGTGAAAGACTGGACG
AGTTTTTACCGCAGGAAGTGAGGCGTATTCTGGTCGGCCGTTATGAGATACGTTACCAGGTGCAGCCGGCAACGATTTAT
GTTCTGCGTATTTGGCATACCCGCGAAGAGCGATAG

Antitoxin

Nucleotide

• Length: 77 bp

>AT187821 NZ_CP068799:c4404561-4404485 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin