Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3988999..3989693 Replicon chromosome
Accession NZ_CP068799
Organism Escherichia coli strain RIVM_C017887

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag JNN46_RS19090 Protein ID WP_001263500.1
Coordinates 3988999..3989397 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag JNN46_RS19095 Protein ID WP_000554758.1
Coordinates 3989400..3989693 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN46_RS19065 3984364..3984822 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
JNN46_RS19070 3985083..3986540 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
JNN46_RS19075 3986597..3987211 - 615 WP_000602124.1 peptide chain release factor H -
JNN46_RS19080 3987208..3988347 - 1140 WP_000521561.1 RNA ligase RtcB family protein -
JNN46_RS19085 3988537..3988989 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
JNN46_RS19090 3988999..3989397 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JNN46_RS19095 3989400..3989693 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JNN46_RS19100 3989745..3990800 - 1056 WP_001226168.1 DNA polymerase IV -
JNN46_RS19105 3990871..3991656 - 786 WP_000207578.1 putative lateral flagellar export/assembly protein LafU -
JNN46_RS19110 3991628..3993340 + 1713 Protein_3746 flagellar biosynthesis protein FlhA -
JNN46_RS19115 3993368..3993577 + 210 WP_071778446.1 hypothetical protein -
JNN46_RS19120 3993660..3994157 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage blaOXA-48 sfaB / sfaC / gmhA/lpcA 3954464..4029998 75534


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T187817 WP_001263500.1 NZ_CP068799:c3989397-3988999 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T187817 NZ_CP090028:c2774294-2774198 [Serratia ureilytica]
AACAAGCCCTGCATTAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT187817 WP_000554758.1 NZ_CP068799:c3989693-3989400 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT187817 NZ_CP090028:2774198-2774338 [Serratia ureilytica]
AAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TTAATGCAGGGCTTGTTCAGCCGTGCACTTTAAGAGTAGCCTACCGCGCCAGTTTTGCCAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References