Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 608600..609399 Replicon chromosome
Accession NZ_CP068799
Organism Escherichia coli strain RIVM_C017887

Toxin (Protein)


Gene name yhaV Uniprot ID Q8FDB4
Locus tag JNN46_RS02940 Protein ID WP_000347252.1
Coordinates 608600..609064 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag JNN46_RS02945 Protein ID WP_001296435.1
Coordinates 609064..609399 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN46_RS02910 603601..604035 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
JNN46_RS02915 604053..604931 - 879 WP_001298314.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JNN46_RS02920 604921..605700 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JNN46_RS02925 605711..606184 - 474 WP_001298322.1 PTS N-acetylgalactosamine transporter subunit IIB -
JNN46_RS02930 606207..607487 - 1281 WP_000681930.1 tagatose-bisphosphate aldolase subunit KbaZ -
JNN46_RS02935 607736..608545 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JNN46_RS02940 608600..609064 - 465 WP_000347252.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JNN46_RS02945 609064..609399 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JNN46_RS02950 609548..611119 - 1572 WP_001273939.1 galactarate dehydratase -
JNN46_RS02955 611494..612828 + 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
JNN46_RS02960 612844..613614 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17750.11 Da        Isoelectric Point: 9.4947

>T187796 WP_000347252.1 NZ_CP068799:c609064-608600 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Download         Length: 465 bp

>T187796 NZ_CP090004:820062-820367 [Flagellimonas sp. HMM57]
ATGTTCTTTATTGAGAAAACAATTGAATTTGACAAGTGGTTTAGAAAACTAAAAAAACTGAAAGCCAAGTCAAAAATATT
GTTCAGAATTCAAAAGATTCAAAGCAACGGACATTTTGGAGATTGTAAAGCTGTTGGTGATGGAATTCGAGAAAAAGGGG
TAAATTTTGCTAAAGGCTATCGTGTTTACTTCAAAGAGAAAAAGGGGAAAATAGTTATTTTACTTATTGGAGGAGATAAA
TCCACACAGCAAAAGGACATCTTGAAAGCAAAAGATATTTGGAAAAACTTAATAGATAAAAAATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT187796 WP_001296435.1 NZ_CP068799:c609399-609064 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT187796 NZ_CP090004:820364-820648 [Flagellimonas sp. HMM57]
ATGAAAACCTCAAAATTTGATATAGCAGATTATCTGGACAGTAAAGAAATGATTGCCGAATACTTGAACACGGTTTTGGA
GGAAGGCGATAACGCAGATATAATCAATGCCATCGGACATGTTGCTAAGGCAATAGGAATGACCAAAATATCCGAAGAAA
CTGGATTGAGCAGACCCAGTCTCTACAAAGCTTTATCGGATGGGGCCAAACCTCAATTTGCAACGGTAATAAAGGTTTTG
AAAGCTATTGGGGGGAAAATTCAAGTAAACCCTATATCTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q8FDB4

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References